Inducing and Screening of High Producing Pectinase Aspergillus niger Strain  

高效产果胶酶的黑曲霉菌种的诱变及筛选(英文)

作　　者：屈二军[1] 李文建[1] 刘云肖[1] 陈兰英[1] 赵桢[1] 

机构地区：[1]河南城建学院生物工程系,河南平顶山467036

出　　处：《Agricultural Science & Technology》2010年第4期22-23,29,共3页农业科学与技术（英文版）

摘　　要：[Objective]The aim was to induce and screen the high producing pectinase Aspergillus niger Strain based on the original preservation strains.[Method]The original strain was induced by ultraviolet,and the highst enzyme activity and cultivated time were detected through the inspection of transparent circle and enzyme activity determination of flask fermentation.[Result] The enzyme activity of strain D1-4 achieved its highest after cultivated for 96 h in suitable conditions,which was 141.13 U/ml.[Conclusion] The induced strain D1-4 had the strong ability of producing pectinase.[目的]以原有保藏菌株经诱变并筛选出高产果胶酶的黑曲霉菌种。[方法]涂布平板法选取透明圈和菌落直径较大者作为出发菌株。采用紫外线进行菌种诱变,选择致死率在70%～80%范围内的诱变后菌株进行初筛和复筛。经透明圈检测和摇瓶发酵酶活检测确定其最高酶活和培养时间。[结果]D1-4菌种在适宜培养基中培养96h酶活性最高,达141.13U/ml,比出发菌株酶活提高了1倍。[结论]诱变菌株D1-4有较强分泌果胶酶的能力。

关 键 词：Aspergillus niger PECTINASE Enzyme activity 

分 类 号：TQ925[轻工技术与工程—发酵工程]