Bioinformatics Analysis on α Subunit Gene of Phycobiliprotein from Spirulina maxima  

极大螺旋藻藻胆蛋白α亚基基因的生物信息学分析(英文)

作　　者：刘士伟[1] 刘杰[2] 李博生[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]北京林业大学螺旋藻研究所,北京100083

出　　处：《Agricultural Science & Technology》2010年第4期86-89,共4页农业科学与技术（英文版）

基　　金：Supported by the Special Project for Public-welfare Industry of Chinese Forestry (200704025) ;The Key Project of Chinese Ministry of Education (102023)~~

摘　　要：[Objective]The α subunit gene of phycobiliprotein from Spirulina maxima was studied in order to provide a basis for the subsequent study of phycobiliprotein.[Method] Amino acids composition,signal peptides,hydrophobicity/hydrophilicity and trails-membrane topological structure of α subunit gene of phycobiliprotein from Spirulina maxima which registered in GenBank（GenBank AF441177） were analyzed and predicted by the tools of bioinformatic analysis.Meanwhile,phylogenetic tree was constructed based on α subunit gene of phycobiliprotein from Spirulina maxima,and its molecular evolution was also analyzed.[Result]The phycobiliprotein was rich in amino acids,which not only contained 18 kinds of essential amino acids,but also contained some non-essential amino acids like glycine,aspartic acid,etc.;Analysis on signal peptides and trails-membrane topological structure showed that the phycobiliprotein belonged to intracellular protein;Analysis on hydrophobicity/hydrophilicity showed that the phycobiliprotein belonged to hydrophilic protein;Phylogenetic analysis showed that the phycobiliprotein had a high homology with Arthrospira,which reached 99%-100%.[Conclusion]The study provided a certain reference for studying the relationship and interaction between α subunit and β subunit.[目的]对极大螺旋藻藻胆蛋白α亚基进行分析研究,为该蛋白的后续研究提供依据。[方法]用生物信息学分析方法对已经在GenBank上登录的极大螺旋藻藻胆蛋白的α亚基基因(GenBank AF441177)及其翻译的氨基酸序列进行了其组成和相关性质的分析、蛋白质信号肽预测、跨膜结构域的预测及亲水性/疏水性的测定,同时构建了分子进化树,对上述结果进行了分析和探讨。[结果]该蛋白的氨基酸组成丰富,不仅含18种必需氨基酸还含有含有甘氨酸、天冬氨酸等非必需氨基酸;对该蛋白信号肽和跨膜结构域分析表明,其属于胞内蛋白;对该蛋白的亲水性分析表明,其属于亲水性蛋白;进化树分析表明,该蛋白与节旋藻的同源性高,达99%～100%。[结论]该研究为α亚基和β亚基之间的关系和相互作用提供了一定依据。

关 键 词：PHYCOBILIPROTEIN α subunit BIOINFORMATICS 

分 类 号：Q943[生物学—植物学]