水解法提取木聚糖工艺条件的正交实验  

Study on extraction of xylanase by hydrolyzing

作　　者：张红丽[1] 何寿林[1] 

机构地区：[1]武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430074

出　　处：《武汉工程大学学报》2010年第9期23-25,共3页Journal of Wuhan Institute of Technology

摘　　要：玉米芯先经酸预处理,用碱液(NaOH溶液)水解法提取木聚糖.为了提高玉米芯水解法获得木聚糖的提取率,通过单因素实验考察了浸提时间、固液比、碱浓度、浸提温度对木聚糖提取率的影响.通过正交实验对工艺条件进行了优化,最佳工艺条件为:NaOH溶液质量分数6%,固液比1∶21,浸提温度91℃,浸提时间94 m in.各因素对提取木聚糖影响程度依次为碱质量分数>浸提温度>浸提时间>固液比.木聚糖最佳提取率为玉米芯原料的20.3%.Corncob was pretreated first by acid and xylan was extracted by hydrolysis of alkali（NaOH solution）.In order to improve the hydrolysis extractiom rate of xylan from corncob,experiment was done by single factor： the concentration of NaOH,the solid-liquid ratio,the extraction temperature and the extraction time.Through orthogonal experiments designation,the optimal technology of extraction was showed as a concentration of NaOH 6%,solid-liquid ratio 1∶21,91 ℃ for 94 min utes.Various factors on the extraction of xylanase followed in the order of the alkali concentration the extraction temperaturethe extraction time the solid-liquid ratio.The best extractiom rate of xylan was 20.3% of raw material of corncob.

关 键 词：玉米芯 水解法 木聚糖 提取工艺 

分 类 号：TS245.8[轻工技术与工程—制糖工程]