酸黄瓜南方根结线虫病抗性的解剖学及其细胞学研究  

Anatomy and cytology of sour cucumber for its resistance to the root-knot nematode Meloidogyne incognita

作　　者：叶德友[1,2] 王暄[3] 张燕霞 钱春桃[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室,南京农业大学园艺学院,南京210095 [2]甘肃省农业科学院蔬菜研究所,兰州730070 [3]南京农业大学植保学院,南京210095

出　　处：《植物病理学报》2010年第5期495-503,共9页Acta Phytopathologica Sinica

基　　金：国家自然科学基金重点项目(30830079);国家“863”项目(2008AA10Z150);国家科技支撑计划项目(2006BAD13B06-3-5)

摘　　要：为探明酸黄瓜南方根结线虫病抗性的内在机理,以抗病材料酸黄瓜与感病材料北京截头为试材,对南方根结线虫侵入与幼虫发育、根的解剖学及线虫侵入诱导形成的取食位点的细胞学结构进行了比较研究。结果表明,抗病材料酸黄瓜根系中线虫侵入率极显著低于感病材料北京截头(P<0.01),酸黄瓜能够有效抵抗南方根结线虫的侵入;抗、感材料根系中雌雄虫个体比例分别为1∶12和1∶5(P<0.01),抗性反应能够抑制线虫取食和幼虫发育;酸黄瓜根的解剖结构与普通栽培黄瓜相似,酸黄瓜的根不具有结构抗性;抗病反应中细胞发生过敏性坏死,感病反应中无此类现象发生。抗侵入、抑制取食和坏死反应是抗病材料酸黄瓜抗性反应的主要特征。To understand the inherent mechanism of resistance to root-knot nematode,Meloidogyne incognita in sour cucumber,the differences of invasion and development of the nematode,the anatomy of root and cytology of feeding site by nematode induction were compared by using resistant sour cucumber and susceptible Beijingjietou.The results showed that the percentage of root invasion in resistant sour cucumber was significantly lower than that in susceptible Beijingjietou（P〈0.01）.The individual ratio of female and male nematodes was 1∶12 and 1∶5 in resistant and susceptible cucumber roots,respectively（P〈0.01）.The resistant reaction observed in sour cucumber could inhibit nematodes from feeding and juvenile development.The anatomy of the root of sour cucumber was similar to that of ordinary cultivated cucumber.The hypersensitive necrosis of cells occured in resistant reaction,while couldn′t be detected in susceptible one.Limited invasion,inhibi-ted feeding of nematodes and necrosis response of feeding site cells were the main features occured in sour cucumber resistant to M.incognita.

关 键 词：酸黄瓜 南方根结线虫 抗性 解剖学 细胞学 

分 类 号：S642.2[农业科学—蔬菜学]