适于双向电泳分析的大豆花瓣采样方法  

Development of Efficient Sampling Method on Flower Petals for Soybean(Glycine max) Floral Development Proteomic Researches

作　　者：刘珊珊[1] 郑天慧[1] 葛玉君[1] 刁桂珠[1] 姜自芹[1] 曾蕊[1] 吴帅[1] 宋波[1] 李文滨[1] 罗达[2] 

机构地区：[1]东北农业大学农学院/教育部大豆生物学重点实验室,黑龙江哈尔滨150030 [2]中国科学院上海植物生理研究所,上海200032

出　　处：《作物杂志》2010年第5期48-51,共4页Crops

基　　金：国家自然科学基金资助项目(31071440);黑龙江省普通高等学校青年骨干支持计划项目(1155G12);博士后研究人员落户黑龙江科研启动项目(2009HB009);转基因生物新品种培育重大专项子任务:抗病虫转基因大豆新品种培育(2008ZX08004-004)资助

摘　　要：大豆花器小,花瓣中蛋白含量低,花瓣采集是大豆花型发育蛋白质组学研究首先要解决的难题。大豆开花期一般持续大约为30d,花瓣采集不仅需要选择恰当的时期、掌握正确的方法,还需要一定的实践经验和技巧。本研究确定了不同发育阶段的大豆花瓣采集标准;明确了采集用于蛋白质组双向电泳分析的不同发育阶段大豆花瓣所需的工作量;建立了1mm花苞大豆花瓣的采集方法。为大豆花型发育蛋白质组双向电泳分析提供了有效的样品采集方法。There are some unique problems for soybean floral patterning proteomics researches because soybean flower is too small. The first step in soybean floral patterning proteomics researches is to collect sufficient amount of sample since at least 5 g of petals are required for once 2-dimensional gel electrophoresis analysis. Thus at least tens of thousands flowers are required. Therefore, an efficient method for collecting petals is absolutely necessary for proteomic analysis of soybean petal development. In this study, we developed a simple, easy and rapid method for sampling three type petals respectively in order to conduct further proteomic analysis of soybean petal development.

关 键 词：大豆 花瓣 双向电泳 样品采集 

分 类 号：S565.1[农业科学—作物学]