胰腺癌细胞线粒体DNA突变的检测和分析  

Detect and analyze the mutation of mitochondria DNA in pancreatic cancer cell

作　　者：叶方鹏[1] 李荣洲[1] 金培声[1] 蔡佩佩 

机构地区：[1]瑞安市人民医院消化内科,浙江省瑞安325200

出　　处：《中国医师杂志》2010年第9期1202-1204,共3页Journal of Chinese Physician

摘　　要：目的 研究胰腺癌细胞线粒体DNA调控序列（D-loop）区突变情况.方法 用PCR与直接测序相结合的方法,对比分析二株胰腺癌细胞株SW1990,JF-305和1例原代培养的正常胰腺细胞的线粒体DNA D-loop区的突变位点.结果 二株胰腺癌细胞系和正常胰腺细胞的线粒体DNA D-loop区均存在不同程度的点突变,SW1990共检测到8个突变位点,JF-305共检测9个突变位点,其中73位A-G,16223位C-T,16358位C-T这三个突变位点在二株癌细胞和正常胰腺细胞中均检测到,考虑为多态性变化,其中16211位C-T,16311位T-C两个相同的突变位点在二株胰腺癌细胞中均检测到,考虑为特征性突变.结论 胰腺癌细胞线粒体DNA D-loop区具有多态性和突变性,其特征性突变有望成为新的分子标记.Objective To explore the mutation situation of pancreatic cancer cell mitochondria DNA D-loop region. Method PCR and direct sequencing was used to analyze the mutational site of mitochondria DNA D-loop region in two pancreatic cancer cell lines SW1990 and JF-305 and normal primary cultured pancreas cell. Result The point mutations were found in two pancreatic cancer cell lines and normal primary cultured pancreas cell. Eight point mutations were found in SW1990 and 9 point mutations were found in JF-305. Three point mutations （73 site A-G,16223 site C-T, and 16358 site c-T） existed in all two pancreatic cancer cell lines and normal primary cultured pancreas cells, which can be considered as polymorphism. Other two point mutations （16211 site C-T and 16311 site T-C） were only found in two pancreatic cancer cell lines, which can be considered as special mutations. Conclusion The mitochondrial DNA D-loop region of pancreatic cancer cells existed polymorphism and special mutations, and the special mutations might be new molecule marker.

关 键 词：胰腺肿瘤/遗传学 DNA突变分析 线粒体/遗传学 

分 类 号：R735[医药卫生—肿瘤]