影响猪胰脂酶催化油茶籽油与亚油酸甲酯酯交换过程中酯交换量和酰基位移因素的研究  

Influence factors of acyl migration and incorporation degree during porcine pancreatic lipase catalyzed interesterification of oil-tea camellia seed oil and linoleic methyl ester

作　　者：杨国龙[1] 王梦华[1] 王业涛[1,2] 付黎敏[1] 

机构地区：[1]河南工业大学粮油食品学院,郑州450052 [2]嘉祥县嘉冠油脂化工有限公司,山东嘉祥272400

出　　处：《中国油脂》2010年第10期38-41,共4页China Oils and Fats

基　　金：国家自然科学基金项目(31071558);河南工业大学校科研基金项目(06XZ007)

摘　　要：研究了无溶剂体系下猪胰脂酶催化油茶籽油与亚油酸甲酯酯交换反应过程中酯交换量和酰基位移的变化。结果显示,反应时间、酶的水活度、加酶量和反应温度对酯交换和酰基位移都有影响。加酶量和反应温度对酰基位移和酯交换量有直接影响,反应时间和酶的水活度通过影响酯交换速率来影响酰基位移。对猪胰脂酶来说,酯交换时酶的水活度在0.12~0.55之间,加酶量不大于10.0%,温度在40~45℃之间,控制合适的反应时间可使产物甘三酯的酰基交换程度较高（24%左右）,酰基位移较小（小于5%）。The changes of acyl migration and incorporation degree during porcine pancreatic lipase catalyzed interesterification of oil-tea camellia seed oil and linoleic methyl ester in a solvent-free system were studied.The results showed that the factors,such as reaction time,water activity of lipase,lipase loading and temperature,affected the acyl migration and incorporation degree.The lipase loading and temperature affected the acyl migration and incorporation degree directly,the effects of reaction time and water activity of lipase on the acyl migration were done through their influences on incorporation velocity of interesterification.For interesterfication catalyzed by porcine pancreatic lipase,when water activity of lipase was in the range of 0.12 to 0.55,lipase loading was not higher than 10.0%,the temperature was in the range of 40℃ to 45℃,the incorporation degree of the product was higher（about 24%）,and the acyl migration was lower（less than 5%）.

关 键 词：猪胰脂酶 酯交换 酯交换量 酰基位移 

分 类 号：TQ641[化学工程—精细化工] O621.14[理学—有机化学]