莪术醇诱导人胃癌BGC-823细胞凋亡与细胞内活性氧增加的实验研究  

Relationship between reactive oxygen species and apoptosis in human gastic cancer BGC-823 cells induced by curcumol

作　　者：徐立春 刘丽娟 王耀霞 陈海燕 

机构地区：[1]扬州大学中西医结合医学研究所,江苏扬州225001

出　　处：《现代肿瘤医学》2010年第10期1892-1895,共4页Journal of Modern Oncology

基　　金：江苏省卫生厅科研基金(编号:K200511);扬州市-扬州大学资助项目(编号:07047-9)

摘　　要：目的:研究莪术醇抑制BGC-823人胃癌细胞增殖、影响细胞凋亡以及细胞内活性氧(reactive oxy-gen species,ROS)水平变化等生物效应。方法:用MTT法、AO/EB双荧光染色及荧光显微镜、FITC-AnnexinV与PI双染法、流式细胞术检测观察各种生物及形态指标。结果:不同浓度莪术醇在不同时间作用BGC-823,可抑制细胞增殖,并呈浓度、时间依赖性;荧光显微镜观察,经莪术醇作用后,实验组细胞核呈固缩状或圆珠状,可见凋亡小体;人胃癌BGC-823细胞经过不同浓度的莪术醇作用后,凋亡细胞比例明显升高,与对照组比较,差异有显著性(P<0.05):不同浓度莪术醇作用人胃癌BGC-823细胞后,细胞内活性氧水平与莪术醇呈浓度依赖性升高,莪术醇作用24、48h后与对照组比较,有显著性差异(P<0.05)。结论:莪术醇可抑制人胃癌BGC-823细胞增殖及诱导细胞凋亡。莪术醇诱导人胃癌BGC-823细胞凋亡与ROS产生增加相关。Objective：To investigate the effect of curcumol on the proliferation,cell apotosis and intracellular reactive oxygen species（ROS） levels and other biological effects of human gastic cancer BGC-823 cells.Methods： The kinds of guide lines in BGC-823 cells were detected by MTT assay,AO/EB,FITC-Annexin V and PI and flow cytometry methods.Results： Curcumol inhibited cell proliferation of human gastric cancer BGC-823 cells（P〈0.05）,in a dose-and-time-dependent manner.After curcumol treated,the nuclei showed pyknosis-like or bead-like,apoptotic bodies by fluorescene microscope,the proportion of apoptotic cells increased significantly（P〈0.05）.The difference of Ros between control group and treated group,24,48 h treated with carcumol was significant（P〈0.05）.Conclusion： Curcumol can inhibit the proliferation and induce the cell apotosis of BGC-823 cells.The effect of curcumol on BGC-823 cells can induce apotosis which related with increased ROS production.

关 键 词：莪术醇 人胃癌BGC-823细胞 细胞凋亡 ROS A0/EB双荧光标记 流式细胞术 

分 类 号：R73-361[医药卫生—肿瘤] R735.2[医药卫生—临床医学]