多重PCR技术在食品微生物检测中的应用进展  

Application of Multiplex PCR in the Detection of Food Microbes

作　　者：陈诺[1] 唐善虎[1] 岑璐伽[1] 李雪[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《中国畜牧兽医》2010年第10期72-75,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：聚合酶链式反应(polymerase chain reaction,PCR)技术在微生物检测中具有快速、灵敏、操作简单等优点,而多重PCR检测还可以同时扩增多个目的基因,对高效快速检测具有重要意义,已经成为近几年来的研究热点。作者对近来多重PCR技术在食品病原微生物、食品非致病微生物及相关环境食品微生物中应用的研究现状,以及未来在食品检测中的应用展望进行了综述。PCR technique has features of rapid,sensitive and friendly operations in the detection of microorganisms.Multiplex PCR method which can simultaneously amplificate multiple target genes is a very useful method for efficient and rapid detection of food microbes and has become a popular area in food research recently.In this paper,the multiplex PCR applied in the detection of food-born pathogenic microorganism,non-pathogenic microorganism,and food related environmental microorganism were reviewed and its application in the future research for food microbe detections were proposed.

关 键 词：多重PCR 食品 微生物检测 

分 类 号：Q78[生物学—分子生物学]