大豆异黄酮的抗氧化和抗肿瘤活性研究  

Research on Antioxygenic and Antitumor Activities of Soybean Isoflavones

作　　者：徐春华[1] 张治广[2] 谢明杰[3] 

机构地区：[1]大连职业技术学院电气与电子工程技术系,辽宁大连116037 [2]辽宁师范大学化学化工学院,辽宁大连116029 [3]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《大豆科学》2010年第5期870-873,共4页Soybean Science

基　　金：大连市科技计划资助项目(2009E12SF165)

摘　　要：采用邻苯三酚自氧化法和Fenton法,检测大豆异黄酮的抗超氧自由基和羟基自由基的活性,通过MTT比色法检测大豆异黄酮对大鼠肝癌CBRH-7919细胞和小鼠白血病CML-K562细胞增殖的影响来研究大豆异黄酮的抗氧化和抗肿瘤活性。结果表明:大豆异黄酮具有显著的抗氧化和抗肿瘤活性。其中大豆异黄酮对超氧自由基的清除率达到50%时所需要的用量(IC50)为0.14 mg.mL-1;对羟基自由基的清除率达到50%时所需要的用量(IC50)为0.57 mg.mL-1;大豆异黄酮对CBRH-7919细胞生长抑制率达到50%时所需要的用量(IC50)为7.55 mg.L-1;对CML-K562细胞生长抑制率达到50%时所需要的用量(IC50)为10.35 mg.L-1。大豆异黄酮具有显著的抗氧化和抗肿瘤活性。To investigate the bioligical activities of soybean isoflavones, pyrogallol autoxidation method and Fenton method were used to detect whether soybean isoflavones could scaverge hydroxyl free radical and superoxide free radical. MTT assay was used to examine the effect of soybean isoflavones on cell viabilities. The results sbowed that soybean isoflavones exbibited antioxygenic activities,the antitumor and antibacterial activities 50% elimination concentration of soybean isoflavones on su- peroxide free radical and hydroxyl free radical was 0.14 mg · mL-1 and 0. 57 mg · mL-1. Soybean isoflavones could inhibit CBRH-7919 cell and CML- K562 cell growth in a dose-depent manner, 50% inhibitory concentration（IC50） on CBRH-7919 cell was 7.55 mg · L-1 ,and ICs0 on CML- K562 cell was 10.35 mg · L-1. Soybean isoflavones exhibited antioxygenic,antitumor and antibacterial activities.

关 键 词：大豆异黄酮 抗氧化活性 抗肿瘤活性 

分 类 号：R151[医药卫生—营养与食品卫生学]