酶电极法测定肌苷片中肌苷  

Enzyme Electrode for Determination of Inosine in Inosine Tablets

作　　者：孙士青[1] 史建国[1] 王少杰[1] 李秋顺[1] 郑晖[1] 

机构地区：[1]山东省科学院生物研究所山东省生物传感器重点试验室,山东济南250014

出　　处：《山东科学》2010年第5期1-4,共4页Shandong Science

基　　金：863国家攻关生物技术主课题传感器子课题(96-C03-04-08);山东省科学仪器设备升级改造技术专项资助(2008GG2TC01011-22)

摘　　要：以固定化核苷磷酸化酶(EC 2.4.2.1)、黄嘌呤氧化酶(EC 1.2.3.2)与过氧化氢电极构成电流型酶电极,研究了肌苷酶电极分析法的各种分析条件及参数,并与紫外分光光度法对比测定了肌苷片中肌苷含量。酶电极法测定结果与紫外法测定结果之间有较好的一致性。采用酶电极法测定肌苷片中肌苷含量专一性高、成本低、分析速度快。We employed an amperometric enzyme electrode constituted by nucleoside phosporylase, xanthine oxidase and a hydrogen peroxide electrode to determine inosine in inosine tables. The correlation coefficient of such enzyme electrode method and ultraviolet method was 0. 8844, better consistency. Experiment shows that this enzyme electrode method has such positives as high specialization, low costs and rapid analysis.

关 键 词：核苷磷酸化酶 黄嘌呤氧化酶 酶电极 肌苷 肌苷片 

分 类 号：TQ400.72[化学工程] R973.4[医药卫生—药品]