两株真菌的分离及其在石油污染土壤修复中的作用  

Isolation of Two Strains of Fungi and Their Effect on Bioremediation of Petroleum-Contaminated Soil

作　　者：秦晓[1] 唐景春[2] 张清敏[2] 高晶[1] 李德生[1] 

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300384 [2]南开大学环境科学与工程学院,天津300071

出　　处：《农业环境科学学报》2010年第10期1999-2004,共6页Journal of Agro-Environment Science

基　　金：环境污染过程与基准教育部重点实验室开放基金;国家高技术研究发展计划项目(863)(2007AA061201);中国科学院知识创新工程项目(kzcx1-yw-06-03)

摘　　要：从石油污染的盐碱土壤中分离获得2株真菌,并对其生理生化性质进行初步研究。将2株菌扩大培养,制成混合菌剂,通过盆栽试验,以石油烃降解率、脱氢酶活性和土壤的微生物多样性等为指标,研究了不同剂量的混合菌剂对石油污染土壤修复的作用。结果表明,添加菌剂各处理的石油烃降解率、脱氢酶活性和微生物多样性明显高于对照;石油烃降解率随菌剂加入剂量的增大而提高,加入8%的菌剂,70 d石油烃降解率可达63%,是对照组的1.44倍。Two strains of fungi were isolated from petroleum-contaminated saline soil and their physiological characteristics were investigat-ed.The bioremediation of petroleum-contaminated soil was studied using the mixed cultures of the isolated strains.The biodegradation effi-ciency,dehydrogenase activity and microbial diversity of the studied soil were monitored for different treatments in pot experiments.The re-sults showed that compared with the contro（lCK）,addition of mixed microbes notably improved the biodegradation efficiency and dehydroge-nase activity and stimulated both the abundance and diversity of the soil microbe.The biodegradation rate increased with higher inoculation amount of microbial consortium.With addition of 8% microbial mixture,the total concentration of petroleum hydrocarbon decreased by 63% after 70 days of incubation,which was 1.44 times higher than that of CK.

关 键 词：石油降解真菌 石油污染土壤 石油烃降解率 脱氢酶活性 微生物多样性 

分 类 号：X172[环境科学与工程—环境科学]