EGCG对低氧诱导下胃癌SGC-7901细胞增殖及凋亡的影响  

Effects of EGCG on proliferation and apoptosis of human gastric cancer cell line SGC7901 under hypoxia

作　　者：姚静静[1] 王琪[1] 齐晓光[1] 蒋荷[1] 林晓琳[1] 王立夫[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化内科,上海200025

出　　处：《上海交通大学学报（医学版）》2010年第10期1199-1203,共5页Journal of Shanghai Jiao tong University:Medical Science

摘　　要：目的探讨表没食子儿茶素没食子酸酯(EGCG)对低氧培养下人胃癌SGC7901细胞增殖、凋亡的影响及其机制。方法将人胃癌细胞株SGC7901进行传代培养,并采用氯化钴(CoCl2)建立低氧模型,实验设空白对照组(常氧组)、低氧对照组及低氧加不同浓度的EGCG组。分别采用MTT法检测细胞活力;流式细胞仪检测细胞凋亡率;RT-PCR和Western blotting检测细胞低氧诱导因子-1α(HIF-1α)和血管内皮生长因子-A(VEGF-A)的表达。结果低氧条件下,低浓度EGCG短时间内(24 h)对SGC7901细胞生长无明显抑制作用(P>0.05);但随着浓度的升高和作用时间的延长,EGCG可抑制低氧SGC7901细胞的增殖(P<0.01),100μg/mL EGCG作用72 h后,其抑制率可达(76.3±2.9)%。流式细胞仪检测显示,EGCG在低氧环境下可呈时间—剂量依赖性地诱导胃癌细胞凋亡(P<0.05或P<0.01)。EGCG可明显抑制低氧诱导的HIF-1α和VEGF-A蛋白的表达(P<0.05或P<0.01),并下调VEGF-A mRNA表达(P<0.05或P<0.01),但对HIF-1αmRNA的转录无明显影响(P>0.05)。结论低氧条件下,EGCG可抑制胃癌细胞的增殖并诱导其凋亡,其作用机制可能与下调低氧诱导的HIF-1α和VEGF-A的表达有关。Objective To explore effects of epigallocatechin-3-gallate（EGCG） on proliferation and apoptosis of human gastric cancer cell line SGC7901 under hypoxia and its mechanism. Methods The SGC7901 cells were subcultured.Hypoxic model was established by Cobalt Chloride（CoCl2）.SGC7901 cells were divided into different groups： control group,hypoxia control group,and hypoxia combined with different concentrations of EGCG groups.The proliferation of SGC7901 cells in different groups was determined by MTT and the apoptosis was analyzed by flow cytometry（FCM）.Besides,RT-PCR and Western blotting were used to detect the expressions of hypoxia inducible factor-1α（HIF-1α） and vascular endothelial growth factor-A（VEGF-A） in the cells. Results Lower concentrations of EGCG had no obvious effects on the proliferation of SGC7901 cells in short time under hypoxia（P0.05）.But with higher dose or longer time,EGCG could further inhibit the proliferation of SGC7901 cells under hypoxia（P0.01）.And the inhibition rate reached（76.3±2.9）% after treatment with 100 μg/mL EGCG for 72 h.FCM results revealed that EGCG induced the apoptosis of SGC7901 cells in a dose-and time-dependent manner（P0.05 or P0.01）.EGCG could reduce obviously the protein levels of HIF-1α and VEGF-A induced by hypoxia（P0.05 or P0.01）,and down-regulate the expression of VEGF-A mRNA（P0.05 or P0.01）,but had no obvious effect on the transcription of HIF-1α（P0.05）. ConclusionEGCG inhibits the proliferation of SGC7901 cells and induces the apoptosis of these cells under hypoxia.These effects may have relationship with the down-regulation of HIF-1α and VEGF-A,which was induced by hypoxia.

关 键 词：表没食子儿茶素没食子酸酯 低氧 胃癌细胞 凋亡 低氧诱导因子-1Α 血管内皮生长因子-A 

分 类 号：R735.2[医药卫生—肿瘤] Q253[医药卫生—临床医学]