一株精氨酸脱亚胺酶产生菌的筛选及鉴定  

Isolation and identification of an arginine deiminase producing strain

作　　者：刘咏梅[1,2] 倪晔[1,2] 孙志浩[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学 生物工程学院,江苏无锡214122

出　　处：《工业微生物》2010年第5期58-62,共5页Industrial Microbiology

基　　金：国家自然科学基金(No.30900030);教育部新教师基金(20090093120008)

摘　　要：精氨酸脱亚胺酶由于具有成为精氨酸营养缺陷型肿瘤如肝细胞瘤,黑素瘤治疗药物的潜力而被国内外多个科研机构进行研究。本文报道本研究室于无锡锡惠公园土样中筛选得到一株产精氨酸脱亚胺酶的菌株901,并对其进行了形态,生理生化特征分析及16S rRNA基因分析,该菌被鉴定为恶臭假单胞菌(Pseudomonas putida)。Arginine deiminase （ADI）, an arginine degrading enzyme, has been studied as a potential anti-cancer agent for inhibiting arginine-auxotrophic tumors, such as melanomas and hepatocellular carcinomas （HCCs）. In this study, a strain 901 producing ADI activity was isolated from the soil samples collected from Xi-Hui Park. Based on its morphological, biochemical characteristics and 16S rRNA gene sequence analysis, the strain 901 was identified as Pseudomonas putida.

关 键 词：恶臭假单胞菌 瓜氨酸 筛选 鉴定 

分 类 号：TQ925[轻工技术与工程—发酵工程]