反相高效液相色谱法测定婴儿配方奶粉中β-胡萝卜素  

Determination of β-carotene in infant formulas by reversed-phase high-performance liquid chromatography

作　　者：舒森 颜景超[2] 彭灿[1,2] 任国谱[2] 

机构地区：[1]湖南亚华乳业有限公司技术中心,长沙410200 [2]中南林业科技大学食品科学与工程学院,长沙410004

出　　处：《食品科技》2010年第10期267-270,共4页Food Science and Technology

基　　金："十一五"国家科技支撑计划课题(2006BAD04A15)

摘　　要：目的:开发并验证一种简单、重现性好的反相高效液相色谱测定婴儿奶粉中胡萝卜素的方法。方法:碱皂化后,用正己烷提取。采用WatersSunFireC18柱分离,柱温30℃,以甲醇∶三氯甲烷∶异丙醇=70∶25∶5为流动相等度洗脱,流速1mL/min,检测波长为450nm。结果:该方法检测限为0.60μg/100g,重复性和重现性的相对偏差分别为1.59%和3.81%,回收率为96.89%。结论:该方法简便,其精度、回收率、灵敏度都很理想,满足日常分析要求。Objective： A simple and reproducible reversed-phase（RP） high-performance liquid chromatography （HPLC） method for determination of β- carotene in infant formulas was developed and validated. Methods： The method involved saponification and a later extraction of β- carotene with n-hexane. Separation was performed on Waters SunFire C18 column at 30 ℃ using an isocratic mobile phase of hexane-chloroform-isopropyl （70∶ 25∶5, v/v/v） and a flow rate of 1 mL/min. Detection was at 450 nm. Results： The detection limits was 0.60 μg/ 100 g, the relative standard deviations of repeatability and reproducibility were 1.59% and 3.81%, respectively and recovery was 96.89% . Conclusion： The method showed desirable values for precision, recovery and sensitivity, and proved very simple for routine analysis work.

关 键 词：婴儿配方奶粉 Β-胡萝卜素 反相高效液相色谱 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]