橙色红曲菌乳清苷-5'-磷酸脱羧酶基因的克隆  

Cloning of Orotidine-5′-phosphate decarboxylase gene from Monascus aurantiacus

作　　者：王伯华[1] 许杨[1,2] 李燕萍[2] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学中德联合研究院,江西南昌330047

出　　处：《食品工业科技》2010年第11期172-174,共3页Science and Technology of Food Industry

基　　金：国家自然科学基金(30860123)

摘　　要：根据已报道的真菌乳清苷-5′-磷酸脱羧酶(pyrG)氨基酸序列,采用CODEHOP策略设计简并引物,从橙色红曲菌(Monascus aurantiacus)的基因组DNA中克隆得到pyrG基因片段。然后运用PCR法筛选橙色红曲菌Fosmid文库,获得了pyrG基因全长(GenBank登录号:GU723506)。经过blastx比较发现,橙色红曲菌pyrG基因与AspergillusflavusNRRL3357的氨基酸序列同源性最高,达到81%。该基因能够作为筛选标记应用于橙色红曲菌同源转化系统。According to the known Orotidine-5′- phosphate decarboxylase, pyrG gene fragment was cloned from Monascus aurantiacus genomic DNA using degenerate primers designed with CODEHOP strategy. And its complete sequence was obtained by a PCR-based method for screening fosmid library（Accession No.GU723506）. After the blastx comparison,the cloned gene was confirmed that it was Monascus pyrG gene with 81% sequence identity to that of Aspergillus flavus NRRL3357. It can use as a selective marker for Monascus aurantiacus homologus transformation system.

关 键 词：橙色红曲菌 简并引物 pyrG基因 基因克隆 

分 类 号：Q785[生物学—分子生物学]