不同补料发酵方式对重组毕赤酵母高密度培养的影响  

Influence of different feeding strategies on high density culture of recombinant pichia pastoris

作　　者：洒荣波[1] 石贵阳[2] 唐瑜菁[1] 

机构地区：[1]泰山医学院生物科学系,山东泰安271016 [2]江南大学生物工程学院,江苏无锡214036

出　　处：《食品工业科技》2010年第11期210-212,419,共4页Science and Technology of Food Industry

基　　金：泰山医学院青年科学基金项目(2008-2009)

摘　　要：目的:研究5L发酵罐中葡萄糖补料方式对重组毕赤酵母高密度培养的影响,为大规模工业化生产奠定基础。方法:葡萄糖分批发酵阶段结束以后,以不同的流加策略流加补料培养基。结果:分批发酵初始葡萄糖最佳浓度为10g/L;采用间歇补料和恒速流加补料方式培养毕赤酵母,发酵结束后菌体细胞干重分别为33.2g/L和41.5g/L,而采用指数流加补料方式,发酵前期和发酵后期控制比生长速率分别为0.20h-1和0.15h-1,发酵结束后菌体细胞干重可达53.4g/L。结论:采用分阶段控制比生长速率的发酵策略,发酵结束后菌体细胞干重远远大于间歇补料和恒速流加补料发酵策略。Objcetive： To develop the influence of three different feeding strategies on high density culture of recombinant pichia pastoris. Method： Medium was fed in different feeding strategies after batch fermentation ended.Results：The optimum concentration for glucose was 10g/L in batch fermentation.The dry cell weight were 33.2g/L and 41.5g/L respectively in intermission and constant velocity fed-batch fermentation.When the specific growth rate was controlled 0.20h^-1 and 0.15h^-1 prophase and anaphase of the fermentation, the dry cell weight in exponential fed-batch processes was 53.4g/L.Conclusion：The dry cell weight with different specific growth rate was higher than the other two fermentation strategies.

关 键 词：重组毕赤酵母 高密度培养 补料方式 

分 类 号：TS201.3[轻工技术与工程—食品科学]