PTEN基因突变及蛋白表达与胃印戒细胞癌的相关性研究  

Correlation between mutation and expression of PTEN Gene and gastric signet-ring cell carcinoma

作　　者：张瑞[1,2] 邹赛英[1] 王晓英 

机构地区：[1]兰州军区乌鲁木齐总医院病理科 [2]石河子大学医学院 [3]解放军69031部队

出　　处：《现代生物医学进展》2010年第19期3626-3631,共6页Progress in Modern Biomedicine

摘　　要：目的:探讨胃印戒细胞癌组织中PTEN基因突变及其蛋白表达异常与胃印戒细胞癌发生、发展和临床病理特征的相关性。方法:采用聚合酶链反应-单链构象多态性(PCR-SSCP)分析法检测36例胃印戒细胞癌组织和36例正常胃组织中PTEN基因突变情况,同时应用链霉菌抗生物蛋白一过氧化酶(S-P)免疫组化方法检测PTEN蛋白的表达并结合临床病理资料进行分析。结果胃印戒细胞癌组织PTEN的表达36.1%,明显低于正常组织94.4%,二者具有显著性差异(P<0.01);PTEN表达与性别和年龄无相关性(P>0.05),与浸润深度、淋巴结转移有相关性(P<0.05);36例胃印戒细胞癌组织中PTEN外显子5突变3例(8.33%),突变位点相对较集中,显示其突变热点主要位于161、162号密码子。结论:PTEN在胃印戒细胞癌组织中表达降低,与其浸润深度、淋巴结转移密切相关,胃印戒细胞癌中PTEN基因突变是其蛋白表达缺失原因之一。Objective： To investigate the mutation of tumor suppressor gene PTEN and the expression of PTEN protein. investigate the association of the dates with the histopathology classification of gastric signet-ring cell carcinoma; investigate the role of PTEN gene in the mechanism of gastric signet-ring cell carcinoma and evaluate their role in the development of gastric tumor. Methods： Immunohistochemistry （S-P） was used to detect the expression of PTEN protein in 36 case of gastric signet-ring cell carcinoma tissues and their adjacent normal gastric tissues;Use PCR-SSCP and DNA sequencing technology to detect the mutation in exon 5 of PTEN gene in 36 gastric signet-ring cell carcinoma tissues. Results： The rate of the expression of PTEN protein in gastric signet-ring cell carcinoma （36.1%） was lower than that in normal gastric tissues （94.4%）. The rate of PTEN protein correlated significantly with the infiltration depth, lymph node transfer （P〈0.05）, but not significantly with sex and age （P〉0.05）. 3 mutations was detected in 36 gastric signet-ring cell carcinoma tissues. The mutation loci was concentrated in the codon 161,162. Conclusions： The PTEN protein expression diversify may play certain role in the genesis and development of gastric carcinoma and closely related the depth of invasion, lymph node metastasis and clinical stages. Mutations was one resons which bring the difference of protein expression.

关 键 词：PTEN 胃印戒细胞癌 免疫组织化学 突变 

分 类 号：R735.2[医药卫生—肿瘤] Q75[医药卫生—临床医学]