Brazilin Content, Antioxidative and Lipase Inhibition Effects of Sappanwood （Caesa/pinia Sappan） from Indonesia  

作　　者：Irmanida Batubara Mohamad Rafi Siti Sa＇diah M. Agung Zaim Susi lndariani Tohru Mitsunaga 

机构地区：[1]Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga, Bogor 16880, Indonesia [2]Biopharmaca Research Center, Bogor Agricultural University, Taman Kencana, Bogor 16151, Indonesia [3]Department of Applied Biological Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan

出　　处：《Journal of Chemistry and Chemical Engineering》2010年第10期50-55,共6页化学与化工（英文版）

摘　　要：Determination of brazilin by High Performance Liquid Chromatography （HPLC） method and measurement of the antioxidative and lipase inhibition effects in the Caesalpinia sappan wood from various locations in Indonesia is described in this paper. Brazilin was separated from sample matrix using a reversed phase C 18, Shim-pack VP column with the mobile phase in a gradient elution for45 min from 5% to 100% methanol in 0.05% aqueous trifluoroacetic acid. Brazilin in ethanolic extracts ofC. sappan wood was in the range of 5.81 to 24.85 mg/g on dry-weight basis. Antioxidant and lipase inhibition activities expressed by IC50 values from all samples were in the range from 6.60 to 11.53 μg/mL and 50.76 to 203.21 μg/mL, respectively. Antioxidative and lipase inhibition potency of all ethanolic extracts were compared to vitamin C and chloramphenicol, isopropyl methylphenol and tetracyclin, respectively.

关 键 词：Caesalpinia sappan sappanwood BRAZILIN antioxidant activity lipase inhibition activity HPLC. 

分 类 号：TQ925.6[轻工技术与工程—发酵工程] Q945.78[生物学—植物学]