白血病细胞PTEN表达与AKT磷酸化水平相关性研究  

Correlation of Expression of PTEN with AKT Phosphorylation Level in Leukemia Cells

作　　者：张媛玉[1] 刘霆[1] 孟文彤[1] 

机构地区：[1]四川大学华西医院血液科,血液病研究室,四川成都610041

出　　处：《中国实验血液学杂志》2010年第5期1109-1113,共5页Journal of Experimental Hematology

摘　　要：本研究探讨各型白血病pten mRNA及蛋白表达与AKT磷酸化水平的关系及其在白血病发病中的作用,为今后PI3K/AKT通路抑制剂的使用提供依据。128例初发的白血病患者纳入研究,其中AML 61例,ALL 27例,CML24例,CLL16例。正常对照21例。采用RT-PCR和Western blot分别检测Jurkat细胞株、白血病患者骨髓单个核细胞pten mRNA表达,PTEN蛋白及P-AKT蛋白的表达。结果表明:Jurkat细胞株pten mRNA表达和PTEN蛋白表达水平均较正常对照组低。AML组pten mRNA表达量虽低于正常对照组,但差异无统计学意义(p=0.274);ALL、CML、CLL各组患者pten mRNA表达量低于正常对照组,差异有统计学意义(p<0.05)。AML、ALL、CML、CLL各组PTEN蛋白表达量均低于正常对照组,P-AKT蛋白表达量均高于正常对照组,差异有统计学意义(p<0.05)。结论:PTEN的低表达及P-AKT高表达在白血病的发生中可能有重要的作用,PTEN的失活主要发生在蛋白质翻译水平。The study was aimed to explore the correlation of expression of pten mRNA and PTEN protein with AKT phosphorylation levels in various types of leukemia and to elucidate its role in the pathogenesis of leukemia so as to provide some evidence for using PI3K/AKT pathway inhibitors in future.128 de novo leukemia patients were eurolled in this study,including 61 AML cases,27 ALL cases,24 CML cases,and 16 CLL cases.21 volunteers were selected as normal control.The RT-PCR and Western blot were used to assay the expressions of pten mRNA,PTEN protein,and P-AKT protein in Jurkat cells,bone marrow mononuclear cells of patients respectively.The results showed that the expressions of pten mRNA and PTEN protein in Jurkat cells were lower than that in normal control group;the expression of pten mRNA in AML group was lower than that in normal control group,but the difference was not significant（p=0.274）;the expressions of pten mRNA in ALL,CML,CLL each group were lower than that in normal control group,and the difference was significant（p0.05）.Compared with normal control group,the expression of PTEN protein was lower and the expression of P-AKT protein was higher in AML,ALL,CML,CLL each group,the difference were significant（p0.05）.It is concluded that the a lower expression of PTEN protein and higher expression of P-AKT protein may play an important role on leukemia pathogenesis,and inactivation of PTEN protein mainly occurs in the level of protein translation.

关 键 词：白血病 PTEN基因 PTEN蛋白 AKT磷酸化 

分 类 号：R733.7[医药卫生—肿瘤]