曲酸诱导CHO-K1细胞凋亡的研究  

Apoptosis of CHO-K1 Cells Induced by Kojic Acid

作　　者：陈永红[1] 黄夏宁[2] 邹志飞[1] 谷康定[2] 刘慧智[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广东广州510623 [2]华中科技大学同济医学院公共卫生学院,湖北武汉430030

出　　处：《环境与健康杂志》2010年第9期785-787,共3页Journal of Environment and Health

基　　金：广州市科技计划项目(2007Z3-E0381);广东出入境检验检疫局科技计划项目(2005GDK56)

摘　　要：目的研究曲酸诱导细胞凋亡的作用。方法采用0、500、1000、1500、2000、2500μg/ml的曲酸染毒CHO-K1细胞,每种浓度条件下分别培养细胞24、48、72、96h,采用流式细胞仪检测细胞凋亡情况。结果流式细胞仪检测结果显示,细胞培养24、48h,未呈现明显的细胞凋亡现象;细胞培养72h,细胞凋亡也非常微弱,其剂量-效应关系不明显。但细胞培养96h,曲酸剂量与细胞凋亡比例呈现明显的剂量-效应关系,曲酸浓度为0、500、1500、2500μg/ml,凋亡早期的细胞比例分别为0.1%,7.3%,45.4%和62.2%。结论曲酸培养CHO-K1细胞96h呈现明显的诱导细胞凋亡效应,而在染毒早期几乎不呈现诱导效应。Objective To investigate the apoptotic effects of kojic acid on CHO-K1 cells.Methods Flow cytometry detecting apoptosis in CHO-K1cells was used in the present study and CHO-K1 cells were treated with the test substances at the doses of 0,500,1 000,1 500,2 000 and 2 500 μg/ml for 24,48,72 and 96 hours respectively in apoptosis detection.Results Flow cytometric analysis showed that CHO-K1 cells treated with kojic acid for 96 hours went to apoptosis markedly with dose dependent manner.No significant apoptosis occurred in less than 72 hours incubation.Conclusion Kojic acid,used under the present experimental conditions for 96 hours,can induce obvious apoptosis,but almost no apoptotic effects can be observed in early time.

关 键 词：曲酸 细胞凋亡 传代细胞株 

分 类 号：R994.6[医药卫生—毒理学]