响应面法优化仿栗籽油超临界萃取工艺  

Optimization of Supercritical Carbon Dioxide Extraction of Oil from Sloanea hemsleyana Seeds Using Response Surface Methodology

作　　者：麻成金[1,2] 吴竹青[2] 傅伟昌[1,2] 黄群[1,2] 陈功锡[1] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000 [2]吉首大学食品科学研究所,湖南吉首416000

出　　处：《食品科学》2010年第18期196-202,共7页Food Science

基　　金：2009年湖南省高校创新平台开放基金项目(09K089)

摘　　要：以仿栗籽为萃取原料,采用响应面法(RSM)优化仿栗籽油的超临界CO2萃取工艺条件,在单因素试验基础上,设定CO2流量为25kg/h、原料粉碎度为40目,然后选取萃取压力、萃取温度、分离温度和萃取时间为影响因子,以仿栗籽油得率为响应值,应用Box-behnken中心组合试验设计建立数学模型,进行响应面分析。结果表明,超临界CO2萃取仿栗籽油的优化工艺条件:萃取压力31MPa、萃取温度47℃、分离温度34℃、萃取时间72min,在此优化条件下,仿栗籽油得率为48.57%。对仿栗籽油的脂肪酸组成进行GC-MS分析,结果表明,仿栗籽油中富含不饱和脂肪酸,其中油酸和亚油酸含量分别为35.17%和19.76%。Response surface methodology（RSM） was applied to optimize the extraction conditions of oil from Sloanea hemsleyana seeds.According to single factor investigations,extraction pressure,temperature and duration and separation temperature were the most important factors affecting oil yield,and carbon dioxide flow rate of 25 kg/h and material particle size of 40 mesh were optimal for oil extraction from Sloanea hemsleyana seeds.A mathematical model with oil yield as a response to extraction pressure,temperature and duration and separation temperature was established using Box-Behnken central composite experimental design.This was followed by response surface analysis.It was found that the optimal process conditions for oil extraction from Sloanea hemsleyana seeds were as follows：extraction pressure 31 MPa;extraction temperature 47℃;separation temperature 34℃;and extraction duration 72 min and that the resultant oil yield was 48.57%.Meanwhile,GC-MS analysis of fatty acid composition of the extracted oil was conducted,and the results showed that the oil was abundant in unsaturated fatty acids contained 35.17% of oleic acid and 19.76% of linoleic acid.

关 键 词：仿栗籽油 超临界CO2萃取 工艺条件 响应面分析 GC-MS 

分 类 号：TS225.6[轻工技术与工程—粮食、油脂及植物蛋白工程] TS224.4[轻工技术与工程—食品科学与工程]