可见分光光度法测定刺山柑花蕾中总黄酮含量  

Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L.Buds

作　　者：姜秀娟[1,2] 唐金成[2] 白红进[1,2] 

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《食品科学》2010年第18期252-254,共3页Food Science

基　　金：新疆生产建设兵团科学技术局政策引导项目(2006YD29);塔里木盆地生物资源保护利用兵团重点实验室开放项目(BR0609)

摘　　要：通过不同方法提取刺山柑花蕾中总黄酮,以芦丁为标准品,NaNO3-Al(NO3)3-NaOH体系显色,可见分光光度法测定其含量,检测波长为500nm。结果表明,甲醇回流法提取效果最好,在0～32mg/L(r=0.9997)范围内线性关系良好,平均加标回收率为100.3%,RSD=0.98%(n=6),测得样品中总黄酮平均含量为1.13%。该法操作简便、结果准确,可用于总黄酮的含量测定。Different extraction methods were coupled with visible spectrophotometry to develop a simple and accurate method for quantifying total flavonoids in Capparis spinosa L.buds.The detection wavelength was set at 500 nm.Rutin was used as the standard sample and NaNO3,Al（NO3）3 and NaOH was used to compose the coloration system.Results indicated that the methanol reflux method exhibited the highest extraction rate for total flavonoids.A good linear relationship was observed in the range of 0 to 32 mg/L（r = 0.9997） and average recovery rate was 100.3% with a relative standard deviation of 0.98%（n=6）.The average content of total flavonoids in the samples from Kuche county,Xinjiang autonomous region was 1.13%.

关 键 词：刺山柑花蕾 总黄酮 分光光度法 含量测定 

分 类 号：O657.32[理学—分析化学]