PCR方法快速检测食品中幽门螺杆菌  

Rapid PCR Detection of Helicobacter pylori in Food

作　　者：史艳宇[1] 刘金华[2] 安伟[1] 李媛媛[1] 王莹[1] 朱颖[1] 王伟[1] 刘俊会[1] 

机构地区：[1]吉林省产品质量监督检验院,吉林长春130022 [2]吉林出入境检验检疫局,吉林长春130062

出　　处：《食品科学》2010年第18期255-257,共3页Food Science

基　　金：国家质量监督检验检疫总局项目(2008QK059)

摘　　要：目的:建立一种快速、特异、灵敏的幽门螺杆菌检测方法。方法:以尿素酶基因序列为靶位点设计引物,进行PCR扩增,建立幽门螺杆菌PCR检测方法。以食品中常见致病菌作参考菌株作特异性检测,分别对幽门螺杆菌菌悬液及鲜牛奶中幽门螺杆菌菌悬液进行梯度稀释,提取DNA后做灵敏度检测。结果:PCR方法能够有效对幽门螺杆菌进行快速检测,具有较强的特异性,灵敏度较高(8CFU/mL)。结论:该方法特异性强,灵敏度高,可以快速、准确检测食品中污染的幽门螺杆菌。The contamination of Helicobacter pylori is considered as a serious problem to impair public health in both developed and developing countries.In order to establish a fast,specific and sensitive method to detect the contamination of Helicobacter pylori in food,PCR method was used to detect the DNA of Helicobacter pylori based on urease gene.Results indicated that this method was highly specific to Helicobacter pylori and sensitive and presented a limit detection of 8 CFU/mL for Helicobacter pylori.Therefore,this method is applicable to clinical practice,environment control and inspection of exported food.

关 键 词：幽门螺杆菌 尿素酶基因 PCR 检测 

分 类 号：Q939.1[生物学—微生物学]