猪釉基质蛋白的提取及N末端氨基酸序列分析  

The extraction and N terminal amino acid sequence analysis of porcine enamel matrix proteins.

作　　者：束蓉[1,2] 李超伦[1,2] 刘正[1,2] 王洪海[1,2] 陈佩丽[1,2] 刘文[1,2] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科 [2]复旦大学遗传所

出　　处：《口腔医学纵横》1999年第2期67-68,共2页Journal of Comprehensive Stomatology

摘　　要：目的：从猪牙胚提取釉基质蛋白并做氨基酸测序，为进一步研究釉基质蛋白诱导牙周组织再生的生物活性奠定基础。方法：选择乙酸提取法获得釉基质蛋白，采用ＳＤＳ－ＰＡＧＥ电泳和印迹膜测序法分析Ｎ末端氨基酸序列。结果：提取的釉基质蛋白分子量条带主要位于２０ＫＤａ以下，优势条带为２０ＫＤａ、１３ＫＤａ、５ＫＤａ。２０ＫＤａ、５ＫＤａ釉基质蛋白Ｎ末端前１０个氨基酸残基均为：Ｍｅｔ、Ｐｒｏ、Ｌｅｕ、Ｐｒｏ、Ｐｒｏ、Ｈｉｓ、Ｐｒｏ、Ｇｌｙ、Ｈｉｓ、Ｐｒｏ。结论：乙酸提取法可获得典型的釉原蛋白。印迹膜测序法简易、快速、敏感。Objective: Enamel matrix proteins from poricine were extracted and determined for the amino acid sequence in the present article, which lay a foundation for further exploring the activity of inducing regeneration of periodontal tissues. Methods: Acetic acid was selected to extract the enamel matrix proteins. The N terminal amino acid sequence of enamel matrix proteins were determined with SDS-PAGE electrophoretic and blot membrnce analysis. Results: The main bands of molecular weighs of enamel matrix proteins are located below 20KDa, prodominating bands are 20, 13 and 5KDa. N terminal sequence of both 20 and 5KDa are Met, Pro, Leu, Pro, Pro, His, Pro, Gly, His, Pro. Conclusions: The typical amelogenin can be obtained by using acetic acid. Blot Membrane analysis is more simpler, quicker, and sensitiver than other.

关 键 词：釉基质蛋白 氨基酸序列 提取 SDS-PAGE 牙周病 

分 类 号：R781.4[医药卫生—口腔医学]