肾癌EGFR表达和DNA含量检测的临床意义  

Expression of EGFR and DNA ploidy in renal carcinoma

作　　者：郭武[1,2,3] 宋宗禄[1,2,3] 臧美孚 黄秉仁[1,2,3] 曹坚 阎治安 

机构地区：[1]北京民航总医院泌尿外科 [2]北京协和医院泌尿外科 [3]北京市垂杨柳医院泌尿外科

出　　处：《中华泌尿外科杂志》1999年第6期338-339,共2页Chinese Journal of Urology

摘　　要：目的探讨表皮生长因子受体（ＥＧＦＲ）表达和ＤＮＡ倍体数与肾癌发生的关系。方法采用免疫荧光标记和流式细胞术双参数法，检测２５例肾癌瘤体及瘤旁组织胞膜ＥＧＦＲ表达和ＤＮＡ含量。结果瘤体组织ＥＧＦＲ为３６５±２３５，明显高于瘤旁组织的１９１±１５０，Ｐ＜００５；瘤体中ＤＮＡ指数（ＤＩ）异常组ＥＧＦＲ为４５１±２３９，正常组为２４３±１７８，Ｐ＜００５。肾癌组织中ＤＩ异常占６４％，而瘤旁组织仅占２３％，Ｐ＜００５。结论ＥＧＦＲ高表达和ＤＮＡ异常与肾癌的发生相关，肿瘤组织ＥＧＦＲ的表达与ＤＩ异常相关。Objective To elucidate the role of the expression of epidermal growth factor receptor (EGFR) and DNA ploidy in the origination of renal cell carcinoma (RCC). Methods Expression of EGFR and DNA ploidy were assayed in 25 fresh tumor tissus and the autologous normal surrounding tissues by means of immunofluorescence and flow cytomerical dualparameter method. Results The average value of EGFR was significantly higher (36.623.5) than the surrounding tissues (19.115.0) (P<0.05).DNA index (DI) was also higher in the tumor tissues (45.123.9 vs.24.317.8,P<0.05).The rate of abnormal DNA ploidy was 64% in the cancer samples but only 23% in the surrounding tissues (P<0.05). Conclusions The high expression of EGFR is associated with abnormal DI in RCC and both the two are associated with the genesis of RCC.

关 键 词：肾肿瘤 癌 EGFR 流式细胞术 DNA 

分 类 号：R737.11[医药卫生—肿瘤]