革胡子鲶体表粘液抗菌肽的分离与纯化及抑菌活性研究  

Research on the Isolation and Purification of Antimicrobial Peptide in the Surface Mucus of Catfish Body and Its Antimicrobial Activity

作　　者：陈成勋[1] 王晓梅[1] 樊振中 戴伟[1] 王欣[1] 

机构地区：[1]天津市水产生态及养殖重点实验室天津农学院水产科学系,天津300384 [2]天津市西青区水产技术推广站,天津300380

出　　处：《安徽农业科学》2010年第26期14437-14439,14445,共4页Journal of Anhui Agricultural Sciences

基　　金：天津市应用基础及前沿技术研究计划重点研究项目(08JCZDJC19000)

摘　　要：[目的]分离纯化革胡子鲶体表粘液抗菌肽,并分析其抑菌活性。[方法]通过硫酸铵沉淀、Sephadex G-50凝胶层析,对高密度养殖的革胡子鲶体表粘液中的抗菌肽进行了初步的分离及纯化,并分析其抑菌活性。[结果]经Sephadex G-50凝胶层析分离得到2个洗脱峰,经检测这2个洗脱峰对迟缓爱德华氏菌抑制作用最强,对嗜水气单胞菌抑制作用次之,对大肠杆菌的抑制作用最弱;对同种菌而言,洗脱峰2的抑菌效果强于洗脱峰1。通过SDS-聚丙烯酰胺凝胶电泳分离,洗脱峰1显示3条蛋白主带,并以9.5kD的蛋白含量最高,而洗脱峰2中所含蛋白的分子量应小于4.1kD。[结论]为探讨高密度养殖条件下革胡子鲶的抗病机理奠定了基础,同时有望为研制和开发新型的水产饲料添加剂和抑菌药物奠定基础。[Objective] The research on the isolation and purification of the antibacterial peptide in the surface mucus of catfish body and the analysis of its antibacterial activity was conducted.[Method] The antimicrobial peptide in the surface mucus of catfish body,which was high intensively raised,was preliminarily isolated and purified and its antibacterial activity was analyzed with the method of ammonium sulfate precipitation,Sephadex G-50 gel chromatography.[Results] Two elution peaks /absorption bands of the testing material appeared in the method of Sephadex G-50 gel chromatography and they showed the strongest inhibition to Edwardsiella tarda,then,Aeromonas hydrophila and the weakest inhibitory effect on E.coli.The antibacterial effect of elution peak No.2 was stronger than that of elution peak No.1 on same species of bacteria.By SDS-polyacrylamide gel electrophoresis,the elution peak No.1 showed three main protein bands,and the highest content of protein appeared at 9.5 kD,while in the elution peak No.2 the molecular weight of protein should be less than 4.1 kD.[Conclusion] The foundation for the disease-resistant mechanism of clarias under the condition of its high-density raising and the research and development of new aquaculture feed additive and antibacterial drug was laid.

关 键 词：革胡子鲶 体表粘液 抗菌肽 抑菌活性 

分 类 号：S969.4[农业科学—水产养殖]