我国猪瘟病毒兔化弱毒株囊膜糖蛋白E0基因的克隆及序列测定  

Cloning and Sequencing of Envelope Glycoprotein E0 Gene of Hog Cholera Virus Strain C

作　　者：李红卫[1,2] 刘湘涛 李小兵[1,2] 韩雪清 殷震 

机构地区：[1]长春农牧大学 [2]兰州兽医研究所

出　　处：《中国病毒学》1999年第2期169-173,共5页Virologica Sinica

基　　金：国家攀登计划B类项目资助课题

摘　　要：采用异硫氰酸胍一步法从４８０代猪瘟病毒兔化弱毒株（ＨＣＬＶ）脾毒中提取总ＲＮＡ，以该ＲＮＡ为模板，进行反转录，然后采用套式ＰＣＲ扩增出ＨＣＬＶ的囊膜糖蛋白Ｅ０基因，琼脂糖凝胶电泳表明其大小与预计相符。将扩增出的Ｅ０基因克隆到ｐＧＥＭＴ载体中，用自动序列分析仪对其进行序列测定。将测得的序列及推导的氨基酸序列与国外测得的Ｃ株相应序列进行比较，结果发现，它们之间核苷酸序列同源性为９９．０８％，氨基酸序列同源性为９８．４２％。The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies on nucleotide and amino acid sequences were 99.08% and 98.42%, respectively.

关 键 词：猪瘟病毒 兔化弱毒株 囊膜糖蛋白 E0基因 HCLV 

分 类 号：S852.651[农业科学—基础兽医学]