红薯叶多糖的分离纯化及其结构鉴定  

SEPARATION,PURIFICATION AND STRUCTURAL IDENTIFICATION OF POLYSACCHARIDE FROM IPOMOEA BATATAS LAM LEAVES

作　　者：刘捷[1] 张体祥[1] 于立芹[1] 卢奎[1] 李亚萍[1] 

机构地区：[1]河南工业大学化学与化工学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2010年第5期46-50,共5页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南工业大学博士科研基金项目;河南省重大科技攻关项目(0422031200)

摘　　要：研究从红薯叶中提取的水溶性多糖的分离、纯化,单糖组成和结构,为红薯叶的开发利用提供参考.通过正丁醇-三氯乙酸(体积比为20∶1)除蛋白、透析法除小分子杂质后,经Seph-adex G-100葡聚糖凝胶柱分离得到了精制多糖IBL(I).通过凝胶过滤法、薄层层析(TLC)、气相色谱(GC)、紫外光谱(UV)、红外光谱(IR)等分析手段初步确定了其组成和结构.红薯叶多糖IBL(I)为均一组分,相对分子质量为2.63×104,其单糖组成为木糖、甘露糖、葡萄糖,其物质的量的比为0.47∶0.35∶0.18.IBL(I)具有典型的多糖特征吸收峰,其结构中存在β型糖苷键.We studied the separation,purification,monosaccharide composition and structure of water-soluble polysaccharides extracted from Ipomoea batatas Lam leaves so as to provide reference for the development of Ipomoea batatas Lam leaves.The method comprised the following steps： removing proteins by using n-butanol and trichloroacetic acid with the volume ratio of 20∶1;removing small-molecule impurities by dialysis;separating refined polysaccharide IBL（I） by Sephadex G-100 gel column chromatography;and determining the composition and the structure of the polysaccharide IBL（I） by gel filtration,thin-layer chromatography,gas chromatography,ultraviolet spectroscopy,infrared spectroscopy and so on.The results show that the polysaccharide IBL（I） has a homogeneous component with the relative molecular weight of 2.63×10^4,and contains xylose,glucose and mannose with the molar ratio of 0.47∶0.35∶0.18;and polysaccharide IBL（I） has a typical characteristic absorption peak,which identifies that the polysaccharide IBL（I） contains a β-glucosidal bond.

关 键 词：红薯叶 多糖 分离纯化 单糖组成 结构 

分 类 号：TS201.2[轻工技术与工程—食品科学]