曲酸对Vero及CHO-K1细胞毒性作用  

Cyotoxic effects of kojic acid on cell lines

作　　者：陈永红[1] 邹志飞[1] 黄夏宁[2] 谷康定[2] 王岚[1] 刘慧智[1] 

机构地区：[1]广东出入境检验检疫局检验检疫技术中心,广州510623 [2]华中科技大学同济医学院公共卫生学院

出　　处：《中国公共卫生》2010年第11期1467-1468,共2页Chinese Journal of Public Health

基　　金：广州市科技计划项目(2007Z3-E0381)

摘　　要：目的探讨曲酸对传代细胞的毒性效应。方法用不同浓度曲酸染毒Vero及CHO-K1传代细胞株,采用噻唑蓝MTT显色法于不同染毒时间终点观察细胞毒性变化。结果细胞与受试物接触24 h之内,曲酸浓度<750μg/mL,Vero细胞株对曲酸反应与阴性对照无明显差异,但随着剂量加大,毒性反应逐渐增强;随着作用时间延长,曲酸对细胞的毒性不断加大,细胞培养48 h,曲酸半数抑制效应(IE50)为2 000μg/mL;细胞培养>72 h,曲酸IE50≤500μg/mL;改变起始染毒方式,对细胞毒性效应无明显影响;CHO-K1细胞株经>1 000μg/mL曲酸染毒后,培养24 h,出现轻微抑制现象;培养48h,细胞毒性作用明显,培养72,96 h毒性反应最强烈。结论曲酸对受试细胞株具有明显毒性作用,且有明显剂量-效应和时间-效应关系。Objective To probe the cytotoxicity of kojic acid to established cell lines.Methods Two cell lines,Vero cell and CHO-K1 cell,were treated with kojic acid for different time and then methyl-thiazolyl-tetrazolium assay was per-formed to evaluate the survival of the cells.Results Cytotoxic effect for Vero cell with the exposure of kojic acid lower than 750 μg /mL for 24 hours incubation was not observed compared with the negative control,but the dose-dependent and time-dependent response was observed after the exposure more than 24 hours.When cells were treated with kojic acid for 48 hours,50% of inhibition effect（IE50） of cell viability appeared at 2 000 μg /mL,followed by IE50 ≤500 μg /mL for more than 72 hours treatment.Cultured cells incubated with kojic acid simultaneously for 24 hours resulted in no difference in tox-icity.There was a light inhibition in CHO-K1 cells exposed to kojic acid of more than 1 000 μg /mL for 24 hours.With elongation of the cell incubation with kojic acid for 48,72 and 96 hours,obvious cytotoxicity to the cells was observed.Con-clusion Kojic acid has toxic effect on Vero and CHO-KI cell lines with significant dose-and time-response manner.

关 键 词：曲酸 传代细胞株 毒性作用 

分 类 号：R114[医药卫生—卫生毒理学]