多不饱和脂肪酸对阿尔茨海默病大鼠海马亨廷顿蛋白相关蛋白1表达的影响  

Effect of polyunsaturated fatty acid on the expression of Huntingtin associated protein 1 in hippocampus of rat model with Alzheimer＇ s disease

作　　者：杨荣[1] 杨友华[1] 黄丹[1] 杨敏[1] 陈晓青[1] 

机构地区：[1],江汉大学医学院生理教研室,武汉430056

出　　处：《中华行为医学与脑科学杂志》2010年第10期882-884,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：湖北省自然科学基金（2006AB198）

摘　　要：目的 探讨多不饱和脂肪酸二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）对阿尔茨海默病（Alzheimer＇s disease,AD）大鼠海马亨廷顿蛋白相关蛋白1（HAP1）表达的影响和认知退化的预防作用.方法 将健康的SD大鼠40只随机分为对照组、AD组、DHA组和EPA组,每组n=10只.皮下注射D-半乳糖及腹腔注射Alcl3联合建立动物AD模型,用Morris水迷宫实验测定大鼠的认知能力,用ABC法免疫组化染色测定大鼠海马HAP1的表达.结果 EPA和DHA明显改善AD大鼠的空间认知能力;AD大鼠海马各亚区HAP1阳性细胞数均减少;AD组和DHA组海马DG区单位面积HAP1阳性细胞数分别为（28.56±4.23）个和（43.57±6.14）个,差异具有显著性（P＜0.05）.结论 AD大鼠海马中的HAP1表达减少,DHA对AD大鼠认知功能的改善可能与其抑制海马中HAP1表达的下降有关.Objective To study the effect of docosahexaenoic acid （ DHA ） and eicosapentaenoic acid （EPA） on cognition impairment and huntingtin associated protein 1 （ HAP1 ） expression in hippocampus of rat model with Alzheimer＇ s disease（AD）. Methods Forty healthy Sprague-Dawley rats were randomly divided into control group, AD group, DHA group and EPA group on average. Alcl3 was injected intraperitoneally and D-galac tose was injected into subcutaneous to establish the model of rat with AD. ABC method of immunohistochemistry was used to observe the expression level of HAP1 in the hippocampus. Morris water maze was used to study the spatial learning and memory in rats. Results Compared with control group,the HAP1-positive neurons of hippo campus decreased and poorer performance in Morris water maze test was observed in AD group. Compared with AD group, DHA and EPA treatments significantly caused the decreases in escape latency and searching distance in the Morris water maze test. The number of HAP1- positive cells in DG region of DHA group（43.57 ±6.14） increased obviously compared with AD group（ 28.56 ± 4.23 ） （P 〈 0.05 =. Conclusion The immunoreactivities of HAP1 decrease in hippocampus of AD rat. The applications of DHA and EPA in AD rats significantly improve the ability of learning and memory;and the mechanisms may be related to the decrease of HAP1 expressions in hippocampus.

关 键 词：亨廷顿蛋白相关蛋白1 海马 二十二碳六烯酸 二十碳五烯酸 

分 类 号：R749.16[医药卫生—神经病学与精神病学]