CSTR直接使用循环废液进行酒精发酵的研究  

Research on the Use of Recycle Effluent Broth for Continuous Ethanol Fermentation by Bioreactor System Composed of CSTR

作　　者：徐铁军[1] 白凤武[2] 

机构地区：[1]沈阳工业大学石油化工学院,辽宁辽阳111003 [2]大连理工大学生物科学与工程学院,辽宁大连116023

出　　处：《酿酒科技》2010年第11期17-19,22,共4页Liquor-Making Science & Technology

基　　金：国家高技术研究与发展计划(863)项目基金资助(No.2002AA647060)

摘　　要：模拟现有酒精发酵装置工艺流程,建立了一套多级串联釜反应器系统,以脱胚脱皮玉米粉双酶法制备的糖化液为原料,利用自絮凝酵母SPSC01进行了蒸馏废液直接循环条件下酒精连续发酵的研究。在平均发酵时间为25～30 h条件下,发酵终点酒精浓度超过12.5%vol以上,残还原糖和残总糖分别在0.85%和1.25%(w/v)以下,发酵罐的设备生产强度达到3.64 g/L.h。自絮凝颗粒酵母体系不仅可以用于搅拌式反应器,还可以直接使用循环废液进行连续酒精的发酵。An ethanol fermentation system composed of CSTR （Continuous Stirred-Tank Reactor ） had been developed, in which a self-flocculating yeast strain SPSC01 developed by protoplast fusion technology was applied to ferment two-stage enzymatic hydrolyzate sugar of corn powder to produce ethanol. The effects of the complete recycling of ethanol distilled effluent was investigated. In condition of the average fermentation time between 25 - 30 h, the ethanol concentration was above 12.5 %（v/v）, and the content of residual reducing sugar and residual total sugar were below 0.85 % （w/v） and 1.25 % （w/v） respectively. The ethanol productivity of the fermentation system was calculated to be 3.64 g/（L-h）. In conclusion, such self-flocculating yeast technology could be used not only for CSTR but also for direct use of recycle effluent broth for continuous ethanol fermentation.

关 键 词：自絮凝酵母 酒精连续发酵 多级串联釜式生物反应器 废液循环 

分 类 号：TS261.1[轻工技术与工程—发酵工程] TS262.2[轻工技术与工程—食品科学与工程]