EGCG对鼻咽癌CNE-2裸鼠移植瘤放疗的增敏作用及机制  

Sensitization and mechanisms of EGCG on radiotherapy of nasopharyngeal carcinoma xenotransplanted tumor in nude mice

作　　者：何晓松[1] 孔中雨[1] 向秋[2] 黄岚珍[2] 蒋伟[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]桂林医学院科学实验中心

出　　处：《山东医药》2010年第39期25-27,共3页Shandong Medical Journal

基　　金：广西壮族自治区科学技术厅计划项目(0640187)

摘　　要：目的探讨表没食子儿茶素没食子酸酯(EGCG)对鼻咽癌裸鼠移植瘤放疗的增敏作用及可能机制。方法将低分化鼻咽癌CNE-2细胞株接种于20只4～6周龄雄性BALA/C裸鼠皮下,肿瘤长至4～6mm时随机分为对照组、EGCG组、放疗组、EGCG+放疗组。分别采用生理盐水、EGCG、放疗、EGCG+放疗等干预。21天取出肿瘤,计算肿瘤抑制率;HE染色观察肿瘤细胞形态变化;RT-PCR检测瘤组织Caspase-3mRNA表达。结果与对照组相比,其他三组肿瘤抑制率均明显升高,EGCG+放疗组显著高于EGCG组及放疗组(P<0.05);对照组肿瘤细胞生长旺盛,EGCG组、放疗组、EGCG+放疗组可见大量凋亡及坏死细胞,其中以EGCG联合放疗组最明显。Caspase-3mRNA表达均明显升高,而以EGCG+放疗组最为明显(P<0.05)。结论 EGCG能增强鼻咽癌CNE-2裸鼠移植瘤对放疗的敏感性,其机制可能与上调Caspase-3mRNA的表达有关。Objective To explore the sensitization and possible mechanisms of Epigallocatechin-3-Gallate （ EGCG） on radiotherapy of nasopharyngeal carcinoma xenotransplanted tumor in nude mice. Methods Human nasopharyngeal carcinoma CNE-2 were inculated into 4-6 week-old male BALA/C nude mice,then were divided into control group ,EGCG group ,radiotherapy group,EGCG ＋ radiotherapy group when the tumor grew to the size of 4-6 mm,and administered respectively by saline,EGCG,radiotherapy,EGCG ＋ radiotherapy. All mice were killed after 21 days,and tumors were obtained to calculate tumor inhibition ratio; HE staining was used to observe the morphological changes ; and RT-PCR was applied to detect the expression of Caspase-3 mRNA in xenografts. Results Compared with the control group,the tumor inhibition ratio of the other three groups increased significantly,and which of the EGCG ＋ radiotherapy group were significantly higher than that of the EGCG group and radiotherapy group（ P 0. 05） ; tumor cells in the control group grew vigorously,and lots of apoptosis and necrosis cells were observed in the other three groups,especially in EGCG ＋ radiotherapy group. Compared with the control group,the expression of Caspase-3 mRNA in the other three groups increased,especially in EGCG ＋ radiotherapy group （ P 0. 05） . Conclusions EGCG can enhance the sensitivity of radiotherapy to the nasopharyngeal carcinoma CNE-2 xenografts in nude mice and its mechanisms may be related to the increase of Caspase-3 mRNA expression.

关 键 词：表没食子儿茶素没食子酸酯 鼻咽癌 裸鼠移植瘤 放射治疗 CASPASE-3 

分 类 号：R739.6[医药卫生—肿瘤]