酶法制备大米抗氧化肽的蛋白酶筛选  

Enzymatic Preparation of Rice Anti-oxidation Peptide

作　　者：马海乐[1] 刘斌[2] 李树君[1,2] 贾俊强[1] 田万敏[1] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]中国农业机械化科学研究院,北京100083

出　　处：《农业机械学报》2010年第11期119-123,共5页Transactions of the Chinese Society for Agricultural Machinery

基　　金："十一五"国家科技支撑计划资助项目(2006BAD27B06);科技部国际合作项目(2009DFA32000);江苏省2008普通高校研究生科研创新计划资助项目(CX08B-149Z)

摘　　要：通过酶法制备进行大米抗氧化肽蛋白酶的筛选。以大米蛋白为原料、酶解液对DPPH自由基清除率为指标,从风味蛋白酶、中性蛋白酶、胰蛋白酶、复合蛋白酶、碱性蛋白酶、木瓜蛋白酶和胃蛋白酶等7种蛋白酶中,筛选出中性蛋白酶为最佳用酶。通过试验得出最佳的酶解反应条件为:底物质量浓度1.0 g/(100 mL)、加酶量8%、pH值6.0、温度60℃、酶解时间30 min。酶解液稀释15倍对DPPH自由基的清除率为89.45%,酶解液清除DPPH自由基的EC50值为0.217 g/(100 mL)。Protease screening was employed to prepare rice anti-oxidation peptide with enzymolysis method.Scavenging rate of hydrolysate to the DPPH radical was used as an index with rice protein as the raw material.Neutral protease was screened out from the following seven kinds of protease.These kinds of protease were flavor protease,neutral protease,trypsin,complex protease,alkaline protease,papain and pepsin respectively.The optimum hydrolysis conditions obtained from the experiments were as the following： concentration of substrate was 1.0 g/（100 mL）,ratios of enzyme-substrate was 8%,pH value 6.0,60℃ and 30 min of reaction time.After being diluted 15 times,scavenging rate of hydrolysate liquid to the DPPH radical 89.45% could be obtained and EC50 value of hydrolysate scavenging DPPH radical was 0.217 g/（100 mL）.

关 键 词：大米蛋白 抗氧化肽 蛋白酶筛选 DPPH自由基清除率 正交试验 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]