灵芝三萜皂苷(GCTL1)的体外抗氧化作用  

Assessment of in vitro Antioxidant Activity of a Triterpenoidal Saponin from Ganoderma lucidum Fruit Bodies

作　　者：崔月花[1] 章克昌[2] 

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]江南大学教育部工业生物技术重点实验室,江苏无锡214122

出　　处：《食品科学》2010年第19期49-53,共5页Food Science

基　　金：扬州大学博士科研启动基金项目

摘　　要：采用化学模拟体系研究灵芝三萜皂苷(GCTL1)对超氧阴离子自由基、羟自由基、DPPH自由基及过氧化氢自由基的清除能力,测定其还原力和铁螯合力,并与合成的抗氧化剂进行比较。结果表明,GCTL1具有较好的抗氧化作用。GCTL1清除超氧阴离子自由基、羟自由基、DPPH自由基、过氧化氢自由基的IC50分别为:(0.247±0.05)、(0.197±0.002)、(0.09±0.02)、(0.279±0.04)mg/mL;还原力为0.5、铁螯合力为50%时GCTL1质量浓度分别为(0.425±0.01)、(0.180±0.04)mg/mL,并且在一定质量浓度范围内具有剂量依赖效应;GCTL1的总体抗氧化能力高于VC和2,6二叔丁基对甲酚(BHT)。To assess the in vitro antioxidant activity of a triterpenoidal saponin from Ganoderma lucidum,named GCTL1,GCTL1 was tested for its abilities to scavenge superoxide anion,hydroxyl,DPPH and hydroxyl peroxide free radicals,reducing power and ferric-chelating capacity and was compared with BHT and vitamin C,synthetic antioxidants. GCTL1 had interesting antioxidant effect. The IC50 values for scavenging superoxide anion,hydroxyl,DPPH and hydroxyl peroxide were （0.247 ± 0.05）,（0.197 ± 0.002）,（0.09 ± 0.02） mg/mL and （0.279 ± 0.04） mg/mL,respectively,and the concentrations for achieving 0.5 reducing power and 50% ferric-chelating capacity were （0.425 ± 0.01） mg/mL and （0.180 ± 0.04） mg/mL. All these antioxidant properties were dependent on concentration varying in a certain range. Collectively,our findings demonstrate that GCTL1 is better antioxidant than BHT and vitamin C.

关 键 词：灵芝 三萜皂苷 抗氧化活性 清除率 

分 类 号：Q946.83[生物学—植物学]