Eu^3＋标抗原盐酸克伦特罗的时间分辨荧光免疫检测  

Time-resolved Fluoroimmunoassay of Clenbuterol Hydrochloride using Europium Labeled Antigen

作　　者：樊晓博[1,2] 杨金易[2] 高秀杰[2] 吴英松[2] 孙远明[2] 雷红涛[2] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]广东省食品质量安全重点实验室,华南农业大学食品学院,广东广州510642

出　　处：《食品科学》2010年第20期307-310,共4页Food Science

摘　　要：采用时间分辨荧光免疫分析(time-resolved fluoroimmunoassay,TRFIA)建立简便、快速、灵敏的盐酸克伦特罗(clenbuterol hydrochloride,CBL)检测方法。以自制盐酸克伦特罗单克隆抗体包被96孔微孔板作为固相抗体,Eu3+标记抗原与样品中的CBL竞争结合抗体,建立直接竞争荧光免疫分析体系。猪尿、组织样品添加回收率实验表明:方法灵敏度为0.02μg/L,样品回收率为91%～101%,与沙丁胺醇、莱克多巴胺等结构类似物的交叉反应率均小于1%。建立盐酸克伦特罗铕标抗原直接竞争时间分辨免疫检测方法,方法灵敏度高、特异性强,且操作简单,完全可以满足现有实际检测需求。A convenient,rapid and sensitive method has been presented for the determination of clenbuterol hydrochloride （CBL） using the direct competitive time-resolved fluoroimmunoassay （TRFIA）. In the direct competitive assay,the anti-CBL monoclonal antibody was bound on the surface of a 96-cell microplate,and samples containing CBL or the standards competed for the antibody binding sites with Eu3＋ labeled antigen in the wells of microplate. The determinations of CBL in porcine urine and tissue samples showed that the limit of detection was 0.02μg/L,with spike recoveries ranging from 91% to 101%. The cross-reaction rates with other six drugs were less than 1%. The proposed TRFIA method is sensitive and specific,and can be used for the determination of CBL residue in food samples.

关 键 词：盐酸克伦特罗 时间分辨 标记抗原 检测 

分 类 号：X792[环境科学与工程—环境工程]