酶解对鲜葱白中含硫化合物的影响  

Studies on the impact of enzymolysis on the sulfur compounds of fistular onion stalk

作　　者：张耕[1] 周银波[2] 张长弓[2] 付琴琴[2] 刘静幽[2] 马丹[2] 

机构地区：[1]武汉市第一医院制剂中心,湖北武汉430022 [2]华中科技大学同济医学院药学院生药系,湖北武汉430022

出　　处：《中国医院药学杂志》2010年第21期1826-1828,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究鲜葱白经酶解和杀酶2种不同方法处理后其含硫类化合物的差异。方法:设定3个实验组和1个对照组。以大蒜酶解条件为依据,将鲜葱白匀浆处理后,调整pH,控制环境温度,使3个实验组充分酶解;参照鲜蒜杀酶方法,将对照组进行杀酶处理。用气相色谱-质谱联用技术对4组葱白的含硫类化合物种类及含量进行分析,比较其差异。结果:3个实验组含硫类化合物的种类基本相同,主要含有噻吩类化合物。按面积归一化法分别算得其含硫类化合物占总物质的29.59%,27.09%,26.21%;对照组只检测到3种含硫化合物,占总含量的2.98%。结论:实验组与对照组含硫化合物的种类与含量出现明显差异,控制条件充分促进鲜葱白发生酶解,可以明显增加其含硫类化合物的含量。此项研究对中药制剂搏心通的开发具有重要的参考价值。OBJECtIVE To study the differences of sulfur compounds between two groups fistular onion stalk; one has been enzyme hydrolyzed, the other hasn＇t. METHODS Four groups were set up in this study. The enzymolysis conditions of the first three experimental groups were controlled, but killed the enzymes of the control group. Then gas chromatography - mass spectrometry technique was used for the measure of sulfur compounds of four groups, their differences were compared. RE- SULTS The types of sulfur compounds of three experimental groups were basically similar, containing mainly thiophene. By area normalization method, we calculated that the total percent of sulfur compounds was 29. 590//00, 27. 09%, 26. 21 0/40 respec- tively; only three sulfur compounds were detected from the control group, the total content was 2. 98%. CONCLUSION There are significant differences between them. We can significantly increase the contents of sulfur compounds by controlling condi- tions. The study is a good guidance for the development of Boxintong.

关 键 词：葱白 酶解 气相色谱-质谱联用 

分 类 号：R927.2[医药卫生—药学]