PTEN基因联合多西环素对黏液表皮样癌细胞侵袭的抑制作用  

Inhibitory Effects of Combination PTEN Gene with Doxycycline on in Vitro Invasion of Human Mucoepidermoid Carcinoma Cell Line

作　　者：刘斌[1] 徐小方[1] 关素敏[1] 徐海燕[2] 安然[2] 

机构地区：[1]第四军医大学口腔医学院生物学教研室,陕西西安710032 [2]第四军医大学口腔医学院实验动物中心

出　　处：《口腔医学研究》2010年第5期617-619,共3页Journal of Oral Science Research

基　　金：国家自然科学基金资助(编号:30470471)

摘　　要：目的:探讨PTEN抑癌基因联合多西环素对高转移性涎腺黏液表皮样癌细胞系体外粘附、迁移和侵袭的影响。方法:用脂质体将野生型PTEN基因导入人黏液表皮样癌细胞系,再用10mg/L多西环素处理细胞3d,采用MTT比色法测定细胞粘附,划伤愈合实验测定细胞迁移,趋化Transwell法测定细胞侵袭。结果:PTEN基因转染和Dox分别处理黏液表皮样癌细胞导致癌细胞粘附、迁移和侵袭受到不同程度的抑制。PTEN基因转染联合多西环素对癌细胞的抑制作用更强,癌细胞在Metrigel和Fn基质上的粘附抑制率分别为37.4%和70.6%;癌细胞迁移抑制率为60.9%;癌细胞侵袭抑制率为65.1%。结论:PTEN抑癌基因联合多西环素对黏液表皮样癌细胞系体外粘附、迁移和侵袭具有显著的协同抑制效应。Objective：To investigate the role of combination exogenous wild-type PTEN gene with doxycycline on in vitro adhesion,migration and invasion of human mucoepidermoid carcinoma cell line.Methods：The wild-type PTEN tumor suppressor gene or empty vector was introduced into mucoepidermoid carcinoma cell line in vitro,then the cancer cells were treated with doxycycline at dose of 10mg/L for 3days.Cell adhesion was determined by MTT assay.Cell migration was determined by wound-healing assay.Cell invasion was evaluated using chemotactic transwell assays.Results：Cell adhension,migration and invasion of mucoepidermoid carcinoma cell line treated by PTEN gene transfection or Dox were inhibited at different extent.PTEN gene transfection combined with doxycycline showed more inhibitory effects.The inhibitory rate of cell dhension on Metrigel or Fn was 37.4% or 70.6% .The inhibitory rate of cell migration was 60.9% and the inhibitory rate of cell invasion was 65.1% .Conclusion：PTEN gene in combination with doxycycline has significantly synergistic inhibitory effects on in vitro adhesion,migration and invasion of human mucoepidermoid carcinoma cell line.

关 键 词：PTEN基因 多西环素 黏液表皮样癌细胞 粘附 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]