E-cadherin基因突变对膀胱癌侵袭能力影响的研究  

Influence of E cadherin mutation on the invasive ability of bladder tumor cells

作　　者：侯树坤[1] 朱积川[1] 张小东[1] 李鹰[1] 王晓峰[1] 

机构地区：[1]北京医科大学人民医院泌尿外科

出　　处：《中华泌尿外科杂志》1999年第5期261-263,共3页Chinese Journal of Urology

基　　金：国家自然科学基金

摘　　要：目的观察Ｅｃａｄｈｅｒｉｎ基因突变对膀胱癌侵袭力的影响。方法对４０例新鲜膀胱癌标本分别完成体外ｍａｔｒｉｇｅｌ穿膜侵袭实验和Ｅｃａｄｈｅｒｉｎ基因的序列测定。结果病理分级Ⅰ、Ⅱ级者３２例中仅有２例出现Ｅｃａｄｈｅｒｉｎ基因突变，而Ⅲ、Ⅳ级者８例中有４例出现突变，说明Ｅｃａｄｈｅｒｉｎ变化与肿瘤细胞分化之间有明显的相关性。本组６例标本出现Ｅｃａｄｈｅｒｉｎ结构改变，其中４例穿膜侵袭实验阳性，而３４例未发现Ｅｃａｄｈｅｒｉｎ基因改变的标本中，只有７例穿膜侵袭实验阳性。结论Ｅｃａｄｈｅｒｉｎ基因改变对肿瘤细胞的侵袭能力有明显影响。未发现Ｅｃａｄｈｅｒｉｎ基因突变的标本也可能具有侵袭能力，说明在膀胱肿瘤的侵袭过程中存在其它机制。Objective To clarify the influence of E cadherin on the invasive ability of bladder tumor cells. Methods 40 fresh bladder cancer tissues were investigated by matrigel invasion assay and direct sequencing of amplified E cadherin complementary DNA fragments. Results In 32 grade Ⅰ and Ⅱcases,E cadherin mutation was identified in only 2 while it was detected in 4 of 8 grade Ⅲ and Ⅳ cases revealing a positive correlation between E cadherin mutation and the differentiation of tumor cells.E cadherin alteration was detected in 6 cases of which 4 specimens showed invasive ability on matrigel invasion assay.Only 7 of the 34 cases without E cadherin alteration penetrated the matrigel barrier. Conclusions E cadherin mutation can significantly influence the invasive ability of tumor cells.On the other hand,some tumors without E cadherin alteration may also have invasive ability denoting that other mechanisms should be consi dered .

关 键 词：膀胱肿瘤 基因突变 E-CADHERIN 

分 类 号：R737.14[医药卫生—肿瘤]