计算流体力学用于不同桨叶组合的ε-聚赖氨酸发酵  

Application of computational fluid dynamics to ε-poly-L-lysine fermentation of different impeller combinations

作　　者：曹逸[1] 陈旭升[1] 张建华[1] 张宏健[1] 毛忠贵[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《化学工程》2010年第10期177-180,共4页Chemical Engineering(China)

摘　　要：采用计算流体力学(CFD)数值模拟方法研究了2种不同桨叶组合(2个相同六直叶桨组合与上下档位分别为螺旋桨、六直叶桨组合)对5 L气液生物反应器的传质混合效果和剪切力的影响,并将这2种桨叶组合用于实验室规模ε-聚赖氨酸发酵。发酵实验结果表明,不同桨叶组合对微生物发酵特性产生了显著影响。相比1号罐,2号罐的菌体呼吸强度得到显著提高,最大菌体摄氧速率和CO2释放速率分别高出23%和32%;ε-聚赖氨酸合成强度提高了8%;同一时刻菌体干质量相差最大高出40%。The influence of two kinds of different impeller combinations（one impeller combination including two six-straight-blade-turbines,the other including six-straight-blade-turbine lower and propeller upper） on mass transfer,mixing effect and shear stress of 5 L gas-liquid bioreactor was studied with computational fluid dynamics（CFD）.Meanwhile,using these two kinds of impeller combinations in laboratory-scale ε-poly-L-lysine fermentation,the result shows that the different impeller combinations bring significantly different phenomena.Compared with bioreactor 1,bioreactor 2 has a huge improvement in cell respiration,and the oxygen uptake rate（OUR） and carbon dioxide evolution rate（CER） are 23% and 32% higher.Furthermore,the synthesis of strength of ε-poly-L-lysine is increase by 8% and the biggest difference of the biomass dry mass at the same time is 40% higher.

关 键 词：桨叶组合 ε-聚赖氨酸发酵 生物反应器 CFD数值模拟 

分 类 号：TQ922.3[轻工技术与工程—发酵工程]