分批发酵和补料分批发酵生产木霉素的研究  

Batch and Fed-Batch Fermentation for Production of Trichodermin

作　　者：申屠旭萍[1] 俞晓平[1] 

机构地区：[1]中国计量学院生命科学学院,杭州310018

出　　处：《中国生物防治》2010年第4期492-496,共5页Chinese Journal of Biological Control

基　　金：浙江省自然科学基金项目(Y3080238);浙江省重大科技项目(2006C12032)

摘　　要：为了提高木霉素的发酵产量,以哈茨木霉Trichoderma harzianum Rifai菌株LC2为生产菌,在10L发酵罐中对分批发酵和补料分批发酵工艺进行了研究。结果表明,在分批发酵工艺中,在8%接种量、4L/min通气量和200r/min搅拌转速条件下,发酵72h时木霉素产量达到最大值149.6mg/L。在间歇补料分批发酵工艺中,分6次补料,使培养基中各成分的终浓度与分批发酵相同,木霉素的发酵产量提高到182mg/L,比分批发酵提高21.7%。In order to enhance the trichodermin production,batch and fed-batch fermentation of Trichoderma harzianum Rifai LC2 in a 10 L fermenter were investigated.In the batch process,under conditions of inoculum size 8%,aeration rate 4 L/min and rotation speed 200 r/min,the trichodermin yield reached 149.6 mg/L after 72 h fermentation.During the intermissive fed-batch process,the feeding medium was added 6 times separately to make the total amount of various components equal to those added in the batch process,and the yield of trichodermin increased to 182 mg/L,21.7% higher than that obtained in the batch process.

关 键 词：木霉素 哈茨木霉菌 分批发酵 补料分批发酵 

分 类 号：S482.292[农业科学—农药学] TQ920.6[农业科学—植物保护]