蝴蝶兰叶外植体发生褐变超微细胞结构和酚类物质分布观察  

Ultrastructure and Distribution of Phenol in Phalaenopsis Browning Leaf Explants

作　　者：许传俊[1,2] 谭茹芳[1] 陈冬茵[1] 赖艳艳[1] 李玲[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点试验室,广东广州510631 [2]福建省亚热带植物研究所,福建厦门361006

出　　处：《北方园艺》2010年第21期90-92,共3页Northern Horticulture

摘　　要：观察蝴蝶兰叶外植体发生褐变后的细胞亚显微结构发现,细胞膜以及细胞壁扭曲变形甚至断裂,细胞壁降解严重,集聚大量黑色颗粒物质,细胞内出现黑色絮状物质,叶绿体结构破坏。Pauly试剂和亚硝酸钠溶液染色结果表明:褐变外植体存在有较多酚类物质,染色较深。以上结果说明,外植体在褐变过程中,细胞膜完整性被破坏,酚类含量增加促进褐变。In the browning Phalaenopsis explants,cell walls were distorted.The cell membranes as well as the cell walls were twist distorts even breakon.And there were abundant of black pellet substance gather in the degraded cell wall.The chloroplasts were shrinked to deep color structure.The results showed that there a lots of phenol in vascular tissues when the browning explant cell treated with Pauly reagent and 10% NaNO2.The integrity of cellular membrane structure of browning explant was destroyed during explant browning.The increasing of polyphenol content in explant aggravated browning.

关 键 词：蝴蝶兰 外植体 褐变 亚显微结构 酚类 

分 类 号：Q943[生物学—植物学]