Microelectrode array recordings of excitability of low Mg^(2+)-induced acute hippocampal slices  

作　　者：Fan Yang Xinwei Gong Haiqing Gong Puming Zhang Peiji Liang Qinchi Lu 

机构地区：[1]Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200001, China [2]College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Neural Regeneration Research》2010年第20期1548-1551,共4页中国神经再生研究（英文版）

摘　　要：Neuronal connections can be detected by neuronal network discharges in hippocampal neurons cultured on multi-electrodes.However,the multi-electrode-array（MEA）has not been widely used in hippocampal slice culture studies focused on epilepsy.The present study induced spontaneous synchronous epileptiform activity using low Mg2＋artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with MEA.Results showed that burst duration and average number of spikes in a burst were significantly greater in the CA3 compared with dentate gyrus and CA1 areas.In Schaffer cut-off group,CA1 area discharges disappeared,but synchronous discharges remained in the CA3 area.Moreover,synchronous discharge frequency in the Schaffer cut-off group was similar to control.However,burst duration and average number of spikes in a burst were significantly decreased compared with control（P 〈 0.05）.Results demonstrated that highest neuronal excitability occurred in the CA3 area,and synchronous discharges induced by low Mg2＋originated from the CA3 region.Neuronal connections can be detected by neuronal network discharges in hippocampal neurons cultured on multi-electrodes.However,the multi-electrode-array（MEA）has not been widely used in hippocampal slice culture studies focused on epilepsy.The present study induced spontaneous synchronous epileptiform activity using low Mg2＋artificial cerebrospinal fluid on acute hippocampal slices to record hippocampal discharges with MEA.Results showed that burst duration and average number of spikes in a burst were significantly greater in the CA3 compared with dentate gyrus and CA1 areas.In Schaffer cut-off group,CA1 area discharges disappeared,but synchronous discharges remained in the CA3 area.Moreover,synchronous discharge frequency in the Schaffer cut-off group was similar to control.However,burst duration and average number of spikes in a burst were significantly decreased compared with control（P 〈 0.05）.Results demonstrated that highest neuronal excitability occurred in the CA3 area,and synchronous discharges induced by low Mg2＋originated from the CA3 region.

关 键 词：EPILEPSY low-Mg2＋ HIPPOCAMPUS internal circuit-loop microelectrode array 

分 类 号：Q426[生物学—神经生物学] TP212[生物学—生理学]