线粒体基因在大肠癌组织中缺失突变的检测  

Detection of the 4977 bp deletion mutation of mitochondrial DNA in colorectal cancer

作　　者：崔海宏[1] 王滨[1] 韩英[2] 李爱民[1] 魏伟[1] 张宝燕[3] 

机构地区：[1]中国人民解放军第456医院内科,山东省济南市250031 [2]北京军区总医院消化科,北京市100700 [3]中国人民解放军第117医院病理科,江苏省杭州市310013

出　　处：《世界华人消化杂志》2010年第27期2934-2937,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨4977bp大片段线粒体DNA(mtDNA)缺失与肿瘤的关系.方法:收集2008年中国人民解放军北京军区总医院及济南军区第456医院50例外科手术患者的大肠癌(colorectal cancer,CRC)组织和癌旁正常组织标本.采用长距离PCR方法检测50例CRC组织、相应的癌旁正常组织和20例非癌患者正常肠组织中4977bp mtDNA的缺失情况.结果:CRC组织标本中检测到4977bp mtDNA的缺失率为10%(5/50),相应的癌旁组织缺失率为18%(9/50),其中3例CRC组织和癌旁正常组织中4977bp mtDNA均有缺失,6例在CRC组织中未发现却在癌旁正常组织中检测到4977bp mtDNA缺失.20例非癌患者正常肠黏膜组织中4977bp mtDNA片段缺失率为15%(3/20).4977bp mtDNA缺失率具有随年龄增大而升高的趋势,将组织样本分为高年龄组(≥60)和低年龄组(<60).高年龄组4977bp mtDNA缺失率显著高于低年龄组(P<0.01).结论:CRC癌变过程中,线粒体缺失不是导致CRC的主要原因.AIM：To investigate the relationship between the 4 977-bp deletion mutation of mitochondrial DNA（mtDNA4977 deletion mutation） and the development of colorectal cancer.METHODS：Colorectal cancer and canceradjacent neoplastic tissue samples taken from 50 colorectal cancer patients treated at the General Hospital of Beijing Military Command and the 117th Hospital of Chinese PLA in 2008,and normal colorectal tissue samples collected from 20 patients without colorectal cancer were included in this study.The mtDNA4977 deletion mutation in these samples was detected by long PCR.RESULTS：The mtDNA4977 deletion mutation was detected in 10%（5/50） of colorectal cancer samples,18%（9/50） of cancer-adjacent neoplastic tissue samples,and 15%（3/20） of normal colorectal tissue samples.The detection rate of the mtDNA4977 deletion mutation increased with age in all the three types of samples.The detection rate of the mtDNA4977 deletion mutation in high-age（≥60 years） group was signif icantly higher than that in low-age（60 years） group（P 0.01）.CONCLUSION：The mtDNA4977 deletion mutation is not specifi c to colorectal cancer and may not play an important role in the carcinogenesis of colorectal cancer.

关 键 词：线粒体DNA 大肠癌 缺失 突变 

分 类 号：R735.34[医药卫生—肿瘤]