毛细管区带电泳法同时分离测定香兰素和邻位香兰素异构体  

Simultaneous separation and determination of vanillin and o-vanillin by capillary zone electrophoresis

作　　者：陈星[1] 关瑾[1] 王慧泽[1] 李云[1] 史哲[1] 

机构地区：[1]沈阳化工大学,辽宁沈阳110142

出　　处：《色谱》2010年第11期1111-1114,共4页Chinese Journal of Chromatography

基　　金：辽宁省自然科学基金项目(No.20092055);辽宁省教育厅2008年度科研计划项目(No.2008S184)

摘　　要：建立了同时测定香兰素和其异构体邻位香兰素的毛细管区带电泳法(CZE)。考察了缓冲溶液的种类、浓度和pH以及分离电压等因素对分离结果的影响。在缓冲溶液为50mmol/L硼砂-150mmol/L磷酸氢二钠(pH7.5)、分离电压15kV的优化条件下,6min内即可实现分离。香兰素和邻位香兰素在10～240mg/L范围内线性关系良好,相关系数分别为0.9999和0.9997;方法的检出限均为1.0mg/L(信噪比为3);样品的加标回收率为99.4%～101.2%,相对标准偏差为0.19%～0.73%。该方法操作简单、快速,已应用于实际样品的分析,并获得了令人满意的结果。A method for the simultaneous separation and determination of vanillin and o-vanillin by capillary zone electrophoresis（CZE）was developed.The influences of type,concentration and pH of running buffer,and applied voltage on separation were investigated.Under the conditions of 50 mmol/L borax-150 mmol/L disodium hydrogen phosphate（pH7.5）and applied voltage of 15 kV,the vanillin and o-vanillin were separated in 6 min.The method was proved to be robust through verification of accuracy,precision and linearity.The calibration curves of vanillin and o-vanillin showed good linearity in the range of 10-240 mg/L,and the correlation coefficients were 0.999 9 and 0.999 7,respectively.The limits of detection for vanillin and o-vanillin were 1.0 mg/L（S/N =3）.The average recoveries at three spiked levels were 99.4%-101.2%with acceptable relative standard deviations of 0.19%-0.73%.The method has been successfully used for the determination of vanillin and o-vanillin in real samples,and the assay results are satisfactory.

关 键 词：毛细管区带电泳 香兰素 邻位香兰素 同分异构体 

分 类 号：O658[理学—分析化学]