小麦品种Waxy蛋白的鉴定和筛选  

Identification and Screen of Waxy Protein in Wheat

作　　者：姚大年[1] 王新望[1] 刘志勇[1] 刘广田[1] 

机构地区：[1]中国农业大学植物科技学院,北京100094

出　　处：《农业生物技术学报》1999年第1期1-9,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金!39770460;安徽省自然科学基金

摘　　要：鉴定了“中国春”小麦及有关染色体缺体-四体系、中国地方小麦品种“白火麦”及国内外400多份品种（系）的Waxy蛋白亚基类型，并从中筛选出43份不同的Waxy蛋白亚基缺失类型材料，特别是筛选出3份Wx－D1亚基缺失类型材料；分析了二倍体小麦、四倍体小麦和原始六倍体小麦（西藏小麦）的Waxy蛋白带型，进一步明确了在小麦的进化过程中，染色体4AL上的片段曾与7BS发生过相互易位，使原来在7BS上的荷载有Wx－B1基因的片段易位到4AL上；初步探讨了PCR技术在小麦Wx基因鉴定中的应用和Waxy蛋白亚基缺失类型的遗传机理。The types of sub-units of waxy protein in wheat including Chinese Spring (CS), nulli-tetrasomic lines of CS, Chinese Local variety-Baihuo and more than 400 wheat Varieties or lines collected from various countries were identified,43 varieties (or lines ) were the types with one or two sub-units of waxy protein, 3 varieties were the types with null Wx-D1 subunits of waxy protein. The types of subunits of waxy protein of einkorn, emmer and Tibeten wheat were analyzed, and a translocation between 4AL and 7BS chromosome might occur in process of evolution from diploid to hexaploid wheat were verified. The fragment with Wx-B1 gene encoded Wx-B1 protein of 7BS, was translated to 4AL. PCR methods were used for identifying the Wx genes as well as the inheritance of waxy protein were studied.

关 键 词：小麦 品质 淀粉 WAXY蛋白 鉴定 筛选 

分 类 号：S512.102.2[农业科学—作物学]