里氏木霉选择性合成木聚糖酶的研究(Ⅰ)  

STUDY ON THE SELECTIVE PRODUCTION OF XYLANASESBY TRICHODERMA REESEI

作　　者：刘超纲[1,2] 勇强[1,2] 周庆庆 余世袁 

机构地区：[1]中南林学院 [2]南京林业大学化工学院

出　　处：《林产化学与工业》1999年第2期8-12,共5页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金

摘　　要：在一定条件下，里氏木霉可选择性地合成木聚糖酶，选择性合成程度与碳源种类、碳源浓度及碳氮比大小有关。以低纤维素污染的木聚糖为碳源，适当降低碳源的浓度，提高碳氮比可以提高木聚糖酶选择性合成的程度。以玉米芯粗木聚糖为碳源，采用添加碳源和氮源的方法控制发酵过程中的碳源浓度在５ｇ／Ｌ下，碳氮比在１１２以上时，产生的木聚糖酶和纤维素酶酶活分别为３５５Ｕ／ｍＬ、０．２Ｕ／ｍＬ。Xylanases could be produced selectively by Trichoderma reesei strain under definite conditions.The degree of selective production was related to type of carbon source,carbon source concentration,and the ratio of carbon to nitrogen(C/N).It could be proportionally raised by lowering carbon source concentration and increasing the C/N ratio,with xylan mixed with very little amount of cellulose as carbon source.High concentration of xylanases(35.5 U/mL)and low concentration of cellulase (0.2U/mL)with high ratio between both enzymes(177.5)could be obtained in fedbatch fermentation,by controlling carbon source under 5 g/L and C/N ratio over 11.2.

关 键 词：木聚糖酶 选择性 合成 里氏木霉 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]