两菌双酶法由苯丙酮酸生产L-苯丙氨酸  

ENZYMATIC PRODUCTION OF LPHENYLALANINE USING COUPLED\= TRANAMINASE AND ASPARTASE SYSTEM

作　　者：徐虹[1] 滕农 王欣荣[1] 范伟平[1] 欧阳平凯[1] 

机构地区：[1]南京化工大学制药与生命科学学院

出　　处：《南京化工大学学报》1999年第3期23-26,共4页Journal of Nanjing University of Chemical Technology(Natural Science Edition)

基　　金：九五国家科委重点攻关项目

摘　　要：大肠杆菌ＥＰ８－１０经培养后产生高活力转氨酶和低活力的天冬氨酸酶，而Ｅ．ｃｏｌｉＥＡ－１则产生高活力天冬氨酸酶和很低的转氨酶，耦合ＥＰ８－１０中转氨酶和ＥＡ－１中Ａｓｐ酶作用，由苯丙酮酸和富马酸生成Ｌ－苯丙氨酸，该体系最佳条件为：ＥＡ－１和ＥＰ８－１０两种细胞质量比为０．６∶１，ｎ（ＰＰＡ）／ｎ（Ｆｕ）＝１∶１．２（ｍｏｌ比），当ＰＰＡ浓度为０．２４ｍｏｌ／Ｌ时，反应６ｈ，可生成３８．５ｇ／ＬＬ－Ｐｈｅ。E. Coli EP810 can produce transaminase with high activity and aspartase with low activity after culture. While, E. Coli EA1 can produce high aspartase activity and low transaminase activity. With coupled transaminase and aspartase system used, Lphenylalanine was produced from phenylpyruvic acid and fumaric acid, Optimum conditions of coupled system was: cell concentration ratio of 0.61, Substrate concentration ratio (PPA to Fu) of 11.2. When phenylpyruvic acid was 0.24 mol/L, 38.5 g/L Lphenylalanine acid was accumulated after 6 h with 97% conversion rate.\=

关 键 词：苯丙氨酸 转氨酶 天冬氨酸酶 酶法 苯丙酮酸 

分 类 号：TQ922.9[轻工技术与工程—发酵工程] TQ464.7