Enantioselective induction of oxidative stress by acetofenate in rat PC12 cells  被引量:5

Enantioselective induction of oxidative stress by acetofenate in rat PC12 cells

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作  者:Ling Li Fen Hu Cui Wang Xuedong Wang 

机构地区:[1]Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China [2]School of Environmental Science and Public Health, Wenzhou Medical College, Wenzhou 325035, China

出  处:《Journal of Environmental Sciences》2010年第12期1980-1986,共7页环境科学学报(英文版)

基  金:supported by the National Basic Research Program(973) of China (No. 2009CB421603);the National Natural Science Foundations of China(No.20877071);the International Cooperation Project of Wenzhou City(No. H20090023)

摘  要:In a non-chiral environment, the enantiomers of a racemate possessed the identical physico-cbemical properties, but in the biological systems they possessed different activities. Considering that the involvement of oxidative damage has been implicated in the toxicities of various pesticides, this study investigated the possibility of enantioselective oxidative stress and cytotoxicity induction by acetofenate (AF) which contains an asymmetrical center on PC12 cells. The results of the cytotoxicity assay indicated that S-(+)-AF presented more toxic effects than R-(-)-AF and (+)-AF. It also demonstrated that S-(+)-AF possessed the strongest effects in induction of reactive oxygen species (ROS) production, decrease in superoxide dismutase (SOD) and catalase (CAT) activities, and increase in malondialdehyde (MDA) level. These results suggested that AF and its enanfiomers could induce enantioselecfive cytotoxicity in PC12 cells mediated by oxidative stress. Therefore, the assessment in environmental safety and new chiral pesticide development should consider enantioselectivity.In a non-chiral environment, the enantiomers of a racemate possessed the identical physico-cbemical properties, but in the biological systems they possessed different activities. Considering that the involvement of oxidative damage has been implicated in the toxicities of various pesticides, this study investigated the possibility of enantioselective oxidative stress and cytotoxicity induction by acetofenate (AF) which contains an asymmetrical center on PC12 cells. The results of the cytotoxicity assay indicated that S-(+)-AF presented more toxic effects than R-(-)-AF and (+)-AF. It also demonstrated that S-(+)-AF possessed the strongest effects in induction of reactive oxygen species (ROS) production, decrease in superoxide dismutase (SOD) and catalase (CAT) activities, and increase in malondialdehyde (MDA) level. These results suggested that AF and its enanfiomers could induce enantioselecfive cytotoxicity in PC12 cells mediated by oxidative stress. Therefore, the assessment in environmental safety and new chiral pesticide development should consider enantioselectivity.

关 键 词:CHIRAL organochlorine pesticides acetofenate ENANTIOSELECTIVITY CYTOTOXICITY oxidative stress 

分 类 号:Q421[生物学—神经生物学] TQ925.6[生物学—生理学]

 

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