抗黄矮病小麦种质的分子标记  

Molecular Markers of Wheat Germplasms Resistant to BYDV

作　　者：马有志[1] 徐琼芳[1] 辛志勇[1] 陈孝[1] 徐惠君[1] 林志珊[1] 张增艳[1] 杜丽璞[1] 李连城[1] 富田因则[2] 中田升[2] 安室喜正[2] 

机构地区：[1]中国农业科学院作物育种栽培研究所,北京100081 [2]日本鸟取大学农学部育种学研究室

出　　处：《作物学报》1999年第4期433-436,共4页Acta Agronomica Sinica

基　　金："863计划"及国家自然科学基金

摘　　要：应用基因组原位杂交技术分析了抗小麦黄矮病种质的遗传组成,研究表明小麦-中间偃麦草部分双二倍体无芒中4（2n=56）具有40条小麦染色体、5对中间偃麦草染色体、3对小麦/中间偃麦草易位染色体,其中1对是罗伯逊氏易位染色体。结果表明无芒中4与远中5的遗传组成有明显差异,是两种不同类型的材料。抗黄矮病小麦种质F940418、T103均为抗病易位系,易位片段位于小麦染色体的端部,且为小片段易位。应用RAPD技术筛选出与来自L_1（抗黄矮病异附加系的抗黄矮病基因连锁的分子标记AB-01_(1500),为抗黄矮病育种提供了可靠的选择标记。Genetic construction of wheat germplasms resistant to BYDV (Barley Yellow Dwarf Virus) were analyzed by Genome In Situ Hybridization (GISH), the results showed that; (1) Wheat/Thinopyrum intermedium a partial amphiploid Zhong 4 awnless (2n = 56) have 20 pairs of wheat chromosomes, five pairs of Th. intermedium chromosomes and three pairs of wheat/Th. intermedium translocated chromosomes, among which one pair is Robertsonian translocation chromosomes. It's proved that Zhong 4 awnless had different genetic composition from Yuan Zhong 5 and these two materials were different genotypes. (2) Wheat germplasms resistant to BYDV, F940418 and T103-4 both are translocaion lines, the translocation fragments located on the end of the wheat chromosomes and were small. (3) Molecular marker AB-011500 linking to BYDV resistant gene from L1 (Aline addition lines resistant to BYDV) was selected by using RAPD, all of these give us the confident selecting markers for the BYDV resistance breeding.

关 键 词：基因组原位杂交 分子标记 小麦 黄矮病 

分 类 号：S512.103.4[农业科学—作物学] S435.121